My Needle Sterilizatiin tek for big batch Innoculations

Discussion in 'MYCO-TEKS' started by BjornButternut, Jul 28, 2020.

  1. BjornButternut

    BjornButternut Member

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    I do this for the lifespan of each needle or more specifically each syringe. When at the inoculation phase whether it be LC, Spawn Bags, or jars and I am exposing an unopened sterilized needle to atmosphere for the first time (whether it be in-front of a flowhood or in a SAB with proper sterile procedure) I immediately push the end of the needle into the rounded end of an ear plug that is saturated with 70 percent iso. Pushing about halfway through the ear pug lengthwise. Logic here being that the buisness end is now isolated from the atmosphere. Then the flat end of the ear plug is held flush against the SSIP and the needle is pushed into the spawn. Barrel of the needle getting run through the iso and then squegeed by the SSIP on its way in. After injecting I keep the ear plug in contact with the SSIP and remove the needle back into it half way before lifting off the SSIP. I have had incredibly good results with this coupled with proper Sanitary and Sterile lab technique throughout the rest of the procedure. Im curious to here some community discussion on this. And before the up roar begins I regularly incorporate flame sterilization into my procedure. However I have used this on days where I am Noc'ing 50 - 100 cc's of the same innoculate and it has dramatically increased the speed of my workload with a shockingly low contam rate considering the dogma that is flame sterilization.
     
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  2. Nihidao

    Nihidao Well-Known Member

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    No uproar from me at all it makes sense! And is a genius step outside the box .kodos Bjorne .flam sterilized is smart but since iWork in a sab where u have to sterilize outside the box then move In it always worried me .will be trying this thanks.
     
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  3. BjornButternut

    BjornButternut Member

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    Absolutely! Let me know how it goes for you, sometimes cheap ear plugs want to compress alot so being gentle when pushing it in and drawing out help it hold its form. A small piece of a sponge, so long as it has small tight cells works well too!
     
  4. Nihidao

    Nihidao Well-Known Member

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    Next time I do some syringe work I will try it and let u know Bjorne
     
  5. SleepDeprivedad

    SleepDeprivedad Well-Known Member

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    damn man ingenious! But what do rednecks like me and @Nihidao know lol! I hate flaming needle as of course your annealing and weakening the needle and I feel the flame debris may build up over time inside the needle and create more contam issues... this is like a sterile slider sleeve kinda deal right?
    Ear plugs have also been used for self healing ports on jar lids lol who'd of thought!
     
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  6. BjornButternut

    BjornButternut Member

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    Exactly, just taking out the chance the needle can be contaminated by airborne debris. Spores and bacteria, while incredibly light weight and small are incapable of influencing their own motion. Their transport is entirely dependent on the movement of objects that have spores/bacteria on them but more specifically and far more often with respect to myco work they are completely dependent on the movement of air currents. Anytime you introduce heat into an environment you are generating the movement of air, and alot more than people give credit for. Anytime I loght a lighter or torch im just like " well here is a giant column of invisible moving air drawing contam at it from below and around it and showering it above my workspace like old faithful" doesn't so much matter if you've got a flowhood but still

    Edit: yes there are bacterial cells that can move, im solely talking about airborne contam
     
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  7. SleepDeprivedad

    SleepDeprivedad Well-Known Member

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    Never even thought about the airflow a flame would make! You must be a cyintist lol! But really thats smart as a SAB is not really the cleanest, specially mine with too large of holes cut for my tiny arms lol! Keep your ideas coming i want more!
     
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  8. Nihidao

    Nihidao Well-Known Member

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    Anyone who has sat in their living room on a sunny day has seen the airborne dust and pollens and spores floating in the air twinkling in the sunlight.u .have me rethinking my butane torch now lol.
     
  9. BjornButternut

    BjornButternut Member

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    I'll keep em coming, there's a huge flush of golden chantrelles right now locally. Ive been foraging about 10lbs a day. Second flush is gonna be insane, talking like 100lbs within a square mile. But over the next two or three weeks while all those pins grow out a bit im gonna write up some stuff
     
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  10. BjornButternut

    BjornButternut Member

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    Right on the money, brotha!
     
  11. Nihidao

    Nihidao Well-Known Member

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    I find morels but nor much else would kill to find a chanterelle patch lol
     
  12. AlexLPD

    AlexLPD New Member

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    Nice write up, some photos will help to non English speakers like my self.
    Thanks !
    -Alex.
     
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  13. SleepDeprivedad

    SleepDeprivedad Well-Known Member

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    Welcome aboard was wondering if these posts were translated or not!
     
  14. AlexLPD

    AlexLPD New Member

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    I read this on English, yet some parts are difficult to grasp, a picture speaks a thousand words.
    -Alex
     
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  15. SleepDeprivedad

    SleepDeprivedad Well-Known Member

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    lol its even hard sometimes to understand these terms in English
     
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  16. AlexLPD

    AlexLPD New Member

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    Thanks for don't make believe I was all alone on this one.
     
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