P.e. swab to agar questions

Discussion in 'QUESTIONS & FEEDBACK' started by Space.ape, Jul 29, 2020.

  1. Space.ape

    Space.ape New Member

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    this is my first attemt at agar, I swabbed these plates almost 2 weeks ago. does the mycelium look usable? I'm not sure when to make the first transfer. Should I let this grow out more or transfer agar to agar now? Screenshot_20200727-171556_Gallery.jpg
     
  2. Nihidao

    Nihidao Well-Known Member

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    I don’t know if u saw my post on shout space .pdais often wispy so u are good there imo.But it looks like u have some color and a bacterial blotch that’s no good .i would take some samples away from the bad spots soon .andone dish I would soak the transfers in 3% hydrogen peroxide.it will slow the growth down but should help knock out any spores are bacteria from riding along .it won’t kill molds are mycelium so if u get unwanted growth transfer till it’s clean .good luck .feel free to ask any questions.
     
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  3. Nihidao

    Nihidao Well-Known Member

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    How did they take off really fast ? that was a dirty swab but I have cleaned up worse .if u live in a state that we’re ordering spores is legal might want to order from a cleaner vendor .depending on how much time u want to spend on the agar work.Im self taught so that’s just my opinion.
     
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  4. Space.ape

    Space.ape New Member

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    Ordered from a pretty reliable source, most likely contams were from my inexperienced work. just made the first transfer. Still fumbled around a little too much tho. But if they keep transferring using sterile technique it should clean up right?
     
  5. Space.ape

    Space.ape New Member

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  6. Space.ape

    Space.ape New Member

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  7. Ratatoskr

    Ratatoskr Plenipotentiary

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    i wouldn't take from any of those plates. how long have those plates been growing since you swabbed them? are you working in a still air box? do you wear a face mask, gloves, sleeves while working in, if it is, the still air box?


    When taking agar to apply to more agar i would suggest taking from the leading edge of mycelial growth that is not near other growth. depending on how long those plates were incubating you may have not transferred the mycelium that you wanted to the new plates.
     
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  8. Space.ape

    Space.ape New Member

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    about 2 weeks. yes, still air box, gloves, flame etc. I will keep in mind about the leading edge in the future.
     
  9. Nihidao

    Nihidao Well-Known Member

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    U have some work ahead of u but can be done
     
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  10. Space.ape

    Space.ape New Member

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    Any recommendations on how many transfers should be done? I'm not too sure what a "clean" sample looks like. What exactly should I be looking for as far as a successful sample? This is after the first transfer from 1 week ago. It is looking good? Or let it go some more and do more transfers?
     
  11. Space.ape

    Space.ape New Member

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  12. Space.ape

    Space.ape New Member

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  13. SleepDeprivedad

    SleepDeprivedad Well-Known Member

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  14. Space.ape

    Space.ape New Member

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    Using prepoured plates.
     
  15. SleepDeprivedad

    SleepDeprivedad Well-Known Member

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    Lucky! Major fail on my first try and scared to try again lol. Those last 2 look like you could get a good transfer but I have never been able to even get any germinated sooo... but I think if you got the outside right where it almost looks a little rhizomorphic you should be good again I have only read on this never experienced...

    I may be mistaken but doesnt PE take along time to do anything?
     
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  16. Nihidao

    Nihidao Well-Known Member

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    Like @SleepDeprivedad said I think if u grab an outside edge of that one u might be good !it looks clean except for the center there .take from the thickest thread looking edge that’s ryzmorphic wich is usualy the most aggressive.How many is till it looks like clean white growth that smells like clean mushroom bacteria smells sweetish are like vinagar .your nose will tell u as much as looks imo.
     
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  17. Nihidao

    Nihidao Well-Known Member

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    I would guess at two more but u never can tell might be beautiful at 2 might look like crap at 4 ! Lol
     
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  18. Nihidao

    Nihidao Well-Known Member

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    See if u can get a pic with some light behind the dish so the mycelium stands out from the pic I can’t tell if it’s powdery looking ? That will help us a lot ape .Also look at some pics of healthy mush mycelium .That will help u a bunch one u see the dif between what the dif tams look like and healthy tissue.
     
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