Huatla de Jimenez Psilocybe caerulescens var. mazatecorum and Psilocybe zapotecorum

Discussion in 'ADVANCED MYCOLOGY' started by elfstone, Jan 20, 2013.

  1. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    I think the sand in the substrate helps considerably.
     
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  2. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    We just received new spore material from an individual who recently worked with a "chota chine" who resides in the forest outside of Huatla and was able to collect spore material from a leftover Ps. caerulescens (derrumbe). The print impressed as good and spore material has been placed on antibiotic agar as well as regular agar. We will keep you posted on the progress of this new phase of the Huatla project.
     
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  3. azure

    azure myco-devotee Mushroom Doctor

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    I was going to mention this just now, but it appears elf beat me to it. I'd love to get a run-down on some of the basic recommendations for both fruiting substrate and casing layer for caerulescens ... I would love to hear from pinhead, trout, TCO, javadog, future and anyone with experience fruiting this one.
     
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  4. kingpsilo

    kingpsilo Well-Known Member

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    Awesome thread guys !
     
  5. azure

    azure myco-devotee Mushroom Doctor

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    this thread is almost two years old in total, and we're STILL working on huautla-caerulescens!
     
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  6. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    I have no doubt it will pay off.
     
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  7. Titus

    Titus Creative PITA

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    Dedication!
     
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  8. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    Indeed
     
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  9. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    So, having transferred sections from the original, contaminated culture to antibiotic agar, troubles persisted with a fungal contaminant, and so the culture was again transferred to antibiotic/peroxide agar, which is assisting in cleaning it up. Here is the original plate (note the pins forming on the mycelium in the 8 o'clock sector):

    [​IMG]

    And now a photo of the current phase of cleanup:

    image.jpeg

    The plan is to continue isolation of strands of the cleaned mycelium until it is unambiguously fully clean by continued transfer onto antibiotic/peroxide agar. I also am going to attenpt isolation using the pins forming on the original culture. I will attempt to pinch some of the pins off the mycelium growing on the contaminated plate using two scalpels, so as to avoid capturing any underlying contaminants and then transfer them to regular agar. We will succeed with this strain!
     

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  10. azure

    azure myco-devotee Mushroom Doctor

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    The zapotecorum that was previously fruited was about as dirty as this one, I don't remember how long it took to clean - but it was at least three generations
     
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  11. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    Is this still bacteria?
    If it is, I would try to sandwich it between 2 layers of antibiotic agar and try to clean up healthy mycelium.

    But you're almost there!
     
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  12. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    I don't think it is bacteria, it is likely another fungal contaminant that is along for the ride - getting rid of it will take time, patience, and persistence, but if it can be done, we will do our level best to make it happen!
     
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  13. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    Looks like a bacteria tagging along from my perspective.
    Does it have a sour smell?

    Try sandwiching it.
    If it works for Stamets, it should work for you.

    I'm 100% positive that this one can be cleaned.
     
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  14. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    It does, indeed, exude a sour smell. I will attempt the sandwich method. Thanks!
     
  15. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    Sour smell = bacteria.

    But you're obviously suppressing it with antibiotic agar which gives the mycelium a chance to thrive and that
    way you've opened a gap to clean it out for good :dance1:
     
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  16. Titus

    Titus Creative PITA

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    The 'sandwich method', that sounds doable, had not known about it, thank you! Damn, I have a few dishes I am going to use this on.
     
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  17. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    So I presume you mean placing a plug of colonized agar face down on the antibiotic agar? Or are you talking about pouring warm agar over the top of the whole culture?
     
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  18. azure

    azure myco-devotee Mushroom Doctor

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    if pinhead or JD are around, perhaps they can detail their pour-over tek ...
     
  19. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    Stamets doesn't state anything about that but common sense tell me that you pour agar over the culture when it is cold enough not to harm the culture.

    But if it was me, I would try both ways.

    You can simply cut a piece of agar from a clean dish and put it on top of your culture when you transfer it.

    You should be clean and good after a few transfers like this.

    Both agar layers should be antibiotic.
     
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  20. azure

    azure myco-devotee Mushroom Doctor

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    I'm betting just sandwiching will clean this up in 2-3 generations and we will be good to go
     
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