Discussion in 'ADVANCED MYCOLOGY' started by elfstone, Jan 20, 2013.
Sounds good - will proceed!
My thoughts exactly
Well, we now have 10 plates of agar sandwiches going - hopefully at least one of them will be a step in the right direction.
Okay - we have the mycelium now coming out clean between the antibiotic agar sandwich, but I am now wondering if what I have is actually caerulescens mycelium as it has a tendency to turn purple at certain points. It has a neutral taste, nothing foul, but does not taste like psilocybe mycelium, either to me or to Azure. I will continue monitoring it, but it does not have the same characteristics as the Tonila mycelium. Just because I am determined to get a caerulescens to fruit, I will concentrate a fair share of my efforts on the Tonila to avoid possible complete disappointment in arriving at some fruit for all our efforts. Azure still has the original dirty print and perhaps something else can be extracted from it. Stay tuned:
And the questionable culture, with purple colored edges (the holes in the middle were taste tests - tastes completely neutral - no odor):
That looks clean as a whistle.
we opened a plate up, smelled the mycellium - tasted the mycellium ... it was totally neutral in terms of taste and smell ... and then it has that purple bit going on ... no idea
Okay - here are several pictures of clean plates:
Note the purplish mycelium above, which is not on the following plate:
The following is just growing a tad more slowly:
The following has more purple mycelium:
Compare this to a pure Tonila culture:
and a second Tonila:
And for good measure, an ovoidiocystidiata:
Any comments on the purple tint? The Huatla caerulescens mycelium tastes neutral and is certainly a mushroom mycelium - I am hopeful!
You've finally cleaned it up
Can't wait until you grow it out
Could the purple be oxidizing actives?
I don't remember ever having purple. Blue yes but not purple like that. I'm not sure what to say.
One of the cultures shows no purpling at all. The ones that do, I suspect, may be responding to the antibiotic/peroxide in the agar. Here is the one that is not purpling and it appears to be forming primordial at about 4 o'clock:
I plan on transferring it to regular agar here soon and seeing what happens with it.
Here is the Huatla caerulescens strain grown out on a modified PFTek substrate using millet flour:
The purple seems to be a trait of this mycelium; we'll see if it fruits.
To compare, the following is the beginning stages of the Tonila strain on the same substrate (with a tablespoon of white sand added to the 1:1:2 millet flour:water:vermiculite mix for 5 jars:
Two fully colonized modified PFTek millet flour jars, cased with standard peat:caco2:gypsum:5%sand were placed in a WonderBubble with an aquarium airstone placed underneath a humidity layer of perlite:2 liters distilled water. A rubber band secures a cut piece of plastic baggie to keep humidity in and bugs out. The cultures were cased a week ago. A white:blue LED light provides a good amount of 420nm light to initiate pinhead formation in the casing. We shall see what happens:
I dont believe the purpling one is mushroom mycelium,at least not a psilocybe
Maybe lipstick mold or similar,the other one looks promising good luck
I am dubious about the purpling one too as it has no odor, but it behaves like a mushroom mycelium. The non-purpling one above is undoubtedly a caerulescens, Tonila, from spores provided by TCO. I started it to have a known caerulescens to compare with the Huatla dirty print culture.
Looking good elfstone...
I'm pulling up a chair and wishing you the best !!
Nice work !! Can't wait to see what happens..
Well, I am sorry to report that the hoped for Huatla caerulescens mycelium appears to be, after all, something along the lines of a lipstick fungus. It just kind of sat there, and then the purple spots eventually turned black and it was obvious, especially in comparison to the Tonila mycelium, which is doing just great, that we were not successful in isolating a caerulescens culture from the dirty print. It seems to be time for another field expedition to Huatla and gathering of a better quality spore print. Ah well....
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