Huatla de Jimenez Psilocybe caerulescens var. mazatecorum and Psilocybe zapotecorum

Discussion in 'ADVANCED MYCOLOGY' started by elfstone, Jan 20, 2013.

  1. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Sounds good - will proceed!
     
  2. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    My thoughts exactly :)
     
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  3. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Well, we now have 10 plates of agar sandwiches going - hopefully at least one of them will be a step in the right direction.
     
  4. Titus

    Titus Creative PITA

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    Fingers crossed.
     
  5. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Okay - we have the mycelium now coming out clean between the antibiotic agar sandwich, but I am now wondering if what I have is actually caerulescens mycelium as it has a tendency to turn purple at certain points. It has a neutral taste, nothing foul, but does not taste like psilocybe mycelium, either to me or to Azure. I will continue monitoring it, but it does not have the same characteristics as the Tonila mycelium. Just because I am determined to get a caerulescens to fruit, I will concentrate a fair share of my efforts on the Tonila to avoid possible complete disappointment in arriving at some fruit for all our efforts. Azure still has the original dirty print and perhaps something else can be extracted from it. Stay tuned:

    CTC_agar_sandwich.jpg

    And the questionable culture, with purple colored edges (the holes in the middle were taste tests - tastes completely neutral - no odor):

    CTC_Questionable.jpg
     
  6. Professor PinHead

    Professor PinHead Lost in the Tek.... Administrator Mushroom Doctor Cannabis Doctor Supporter

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    That looks clean as a whistle. :)
     
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  7. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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  8. azure

    azure myco-devotee Mushroom Doctor

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    we opened a plate up, smelled the mycellium - tasted the mycellium ... it was totally neutral in terms of taste and smell ... and then it has that purple bit going on ... no idea
     
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  9. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Okay - here are several pictures of clean plates:

    image.jpeg

    Note the purplish mycelium above, which is not on the following plate:

    image.jpeg

    The following is just growing a tad more slowly:

    image.jpeg

    The following has more purple mycelium:

    image.jpeg

    Compare this to a pure Tonila culture:

    image.jpeg

    and a second Tonila:

    image.jpeg

    And for good measure, an ovoidiocystidiata:

    image.jpeg

    Any comments on the purple tint? The Huatla caerulescens mycelium tastes neutral and is certainly a mushroom mycelium - I am hopeful!
     
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  10. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    You've finally cleaned it up :super:

    Can't wait until you grow it out
     
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  11. RhizoDork

    RhizoDork Fake it till you make it

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    Could the purple be oxidizing actives?
     
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  12. Trout

    Trout Closet Mycologist, Literaly! Mushroom Doctor

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    I don't remember ever having purple. Blue yes but not purple like that. I'm not sure what to say.
     
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  13. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    One of the cultures shows no purpling at all. The ones that do, I suspect, may be responding to the antibiotic/peroxide in the agar. Here is the one that is not purpling and it appears to be forming primordial at about 4 o'clock:

    IMG_2018.jpg

    I plan on transferring it to regular agar here soon and seeing what happens with it.
     
    Last edited: Jan 18, 2016
  14. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Here is the Huatla caerulescens strain grown out on a modified PFTek substrate using millet flour:

    image.jpeg
    The purple seems to be a trait of this mycelium; we'll see if it fruits.

    To compare, the following is the beginning stages of the Tonila strain on the same substrate (with a tablespoon of white sand added to the 1:1:2 millet flour:water:vermiculite mix for 5 jars:

    image.jpeg
     
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  15. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Two fully colonized modified PFTek millet flour jars, cased with standard peat:caco2:gypsum:5%sand were placed in a WonderBubble with an aquarium airstone placed underneath a humidity layer of perlite:2 liters distilled water. A rubber band secures a cut piece of plastic baggie to keep humidity in and bugs out. The cultures were cased a week ago. A white:blue LED light provides a good amount of 420nm light to initiate pinhead formation in the casing. We shall see what happens:

    image.jpeg

    image.jpeg
     
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  16. eLShaMukO

    eLShaMukO Moderator Moderator Mushroom Doctor

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    I dont believe the purpling one is mushroom mycelium,at least not a psilocybe
    Maybe lipstick mold or similar,the other one looks promising good luck
     
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  17. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    I am dubious about the purpling one too as it has no odor, but it behaves like a mushroom mycelium. The non-purpling one above is undoubtedly a caerulescens, Tonila, from spores provided by TCO. I started it to have a known caerulescens to compare with the Huatla dirty print culture.
     
    Last edited: Feb 16, 2016
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  18. Viridis420

    Viridis420 New Member

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    Looking good elfstone...

    I'm pulling up a chair and wishing you the best !!
     
  19. kingpsilo

    kingpsilo Well-Known Member

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    Nice work !! Can't wait to see what happens..
     
  20. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Well, I am sorry to report that the hoped for Huatla caerulescens mycelium appears to be, after all, something along the lines of a lipstick fungus. It just kind of sat there, and then the purple spots eventually turned black and it was obvious, especially in comparison to the Tonila mycelium, which is doing just great, that we were not successful in isolating a caerulescens culture from the dirty print. It seems to be time for another field expedition to Huatla and gathering of a better quality spore print. Ah well....
     
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