Reuse Plastic Petri Tek

Discussion in 'MYCO-TEKS' started by FewTrueSeed, Nov 21, 2014.

  1. Titus

    Titus Creative PITA

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    I prefer our glass Petri dishes, but do re-use properly sanitized plastic dishes, and cleaned and sterilized used bags. Waste not, want not, so to speak.
     
  2. FewTrueSeed

    FewTrueSeed Many meiosis Supporter

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  3. GoldiBoi

    GoldiBoi New Member

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    I have never re used a grow bag. This is do-able? I just figured the filter or integrity of the bag might be compromised after multiple pressure cooking cycles.
     
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  4. treewood

    treewood mediocre mycologist Moderator Mushroom Doctor Supporter

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    It def works. I have done it twice. I know people who done it 5 times. I only recommend using them once. (for sterilizing )
     
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  5. FractalXplora

    FractalXplora Master Mycologist

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    good idea, what a waste throwing them out. I just use agar jars now, as it pains me to have to pour plates in a SAB. Such a PITA. :)
     
  6. nomendubium

    nomendubium scraping by, since '97 Expert Identifier

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    15% bleach is way overkill. Bleach causes pits in plastic (reason why you are not allowed to use it on instruments in the medical feild) 15% bleach is over 6500 ppm!
    You only need 400 ppm at the most, since you are soaking them in it. 10% bleach kills nearly all organisms/spores in seconds
    http://ucfoodsafety.ucdavis.edu/files/26437.pdf
     
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  7. Tammy

    Tammy Active Member

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    Is that made from a 3.5% sodium hypochlorite solution?
     
  8. nomendubium

    nomendubium scraping by, since '97 Expert Identifier

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    for some reason I seem to recall reading at the USDA that the average chlorine bleach was 5.25% sodium hypochlorite
     
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  9. Tammy

    Tammy Active Member

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    All our bleach here are 3.5% At one time I had to make a solution for bacterial blotch, the bleach they were using was 10% sodium hypochlorite, so I just used 3 times the amount that were suggested...it worked so great, I use it now every time in my misting bottles. I just don't know how much I should use for 15% bleach if I don't know how much Sodium hypochlorite it contains...
     
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  10. AlexLPD

    AlexLPD New Member

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    Thanks for the idea... I don't like the idea to have to trow away nice plates. And until I have a nice set of cristal plates this seems very necessary.
     
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  11. SleepDeprivedad

    SleepDeprivedad Well-Known Member

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    They think of it all!
     
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