This is not my tek, I seen it somewhere and have modified it the way I like. I started using it in 09 and it works very well. I use malt yeast agar and I use the 4 ounce ball mason jars my lid tek is to flip the lid over so the rubber seal faces up, and that's it the metal and glass will not seal so it gets gas exchange. I also only tighten finger tight. Not loose though Inoculated with your favorite spores or isolated mycelium or whatever. Let colonize fully Next take a sterile syringe with a 16 g needle 18 will work just not that good. Flame sterilize the needle and use it to scrape the mycelium of the agar. You want to make sure that you scrape it so your pieces of mycelium are small enough to fit in the needle. Looks like this. Next take sterilied water, I do two full syringes if you do 3 there weaker but work. Add the water, put the lid on and swirl not to hard because there's no seal.. Suck back into the syringe and use like a bad ass LC.