Growing Lions Mane and keeping it going?

Discussion in 'EDIBLE & MEDICINAL MUSHROOMS' started by BTL99, Aug 31, 2018.

  1. BTL99

    BTL99 Active Member

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    So I want to start growing Lions Mane and I want to be able to keep growing it from an LC or agar.

    Is it as simple as creating the substrate with the pellets and then buying a Lions Mane LC syringe and putting that into grain spawn? Then using that LC syringe and turning that into more LC's or even agar and then creating more substrate/spawn and repeating?

    Am I off base here or what? Any suggestions?

    Thanks.
     
    Last edited: Sep 1, 2018
  2. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    That basically sums it up. Some people love lc's and some people hate them....i say to use whatever works best for you. Make up your bulk sub when the spawn is ready.
     
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  3. PitcherCrab

    PitcherCrab Supa Fresh Supporter

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    Yup! That's about right.

    I'd suggest using some of your LC to make a plate or a few master agar slants which you keep in cold storage. Use those to make new plates. The idea is in order to prevent senescence (genetic degridation) you want to keep the cell line as young as possible. So with that in mind you don't want to grow by continually cloning your fruits.

    A better way is: Master Slant => Plate => Grain Master => Bulk Grain => Fruiting substrate. This will keep your genetics fresh and fruiting well for years. You will need to refresh your slants every once and a while however.
     
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  4. BTL99

    BTL99 Active Member

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    Great input there thanks. I have never tried doing culture slants, but I ordered some tubes and found a tek so I am definitely going to do that.
     
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  5. PitcherCrab

    PitcherCrab Supa Fresh Supporter

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    They’re pretty easy. I always made mine with PDYA and put a popsicle stick in there as well for the mycelium to colonize. I think I heard about that somewhere a long time ago and I’ve been doing it ever since. It’s supposed to help with the longevity of the tube and if necessary you can use a peice of the colonized stick to start the culture if something happens. Get one of those plastic tube racks to help put the correct stant on your tubes. Also its helpful with your labeling to indicate how many transfers you are away from the master slant.

    A lot of folks do it by labeling P0, P1, P2, P3 etc. (the lower P value being closer to the master). So an example of how I would keep a strain of my Pleurotus erengii would be, “PE3 P1 02/9/19” (for a plate one transfer away from the grain master). Senescence really doesn’t really start happening until you are doing a ton of G2G to expand it out before you fruit, but I like to keep track anyway.

    Good luck, and have fun!
     
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