Using non-sterile spore prints

Discussion in 'QUESTIONS & FEEDBACK' started by Swamp Rat, May 21, 2020.

  1. Swamp Rat

    Swamp Rat New Member

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    Are there any ways to sterilize or reduce the risk of contamination from trying to use spores from prints that were taken and stored in open air, non-sterile manner? Thinking of trying to use some prints of 'wild' mushrooms I was using for identification purposes, and innoculating a Ancient Path Naturals Boom Block to see if I can get any results. I am guessing syringe would be easiest, but not sure if its worth it if contamination is anything more than a low-moderate risk. TIA
     
  2. SleepDeprivedad

    SleepDeprivedad Active Member

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    Hello and welcome! Do you have any experience with agar? From what I've heard Agar from spore is the best for cleaning up the baddies. You could also do up small grain jars like 8 or 12oz do like 4 and if they all colonized you can do grain to grain. If you want to knock something up you can take a colonized grain jar and using clean syringe and water spray into jar and shake then suck out slurry with syringe... forgot what it's called but this guys Alvin does it on YouTube. Hes the one with jesus's face as his profile pic... there are lots of options as spore prints are technically never clean due to being exposed to air while fruiting. Hope I gave you some ideas enjoy!
     
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  3. Swamp Rat

    Swamp Rat New Member

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    Thanks! I have no experience with agar. My only experience is from about 12 years ago using PF Tek and commercial syringes - successfully. I recently got back into foraging for edibles so starting to consider the possibility of some home cultivation. Will have to do some homework about the agar so thanks for the recommendation!
     
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  4. SleepDeprivedad

    SleepDeprivedad Active Member

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    For sure anything to help another struggler out! Check this tek, people say it has been very successful especially if you dont have flow hood or SAB.

    No pour agar
    http://mycotek.org/index.php?threads/276/
     
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