Hey all, new to the forum but wanted to share my current project. Small scale, mini tubs: one is unmodified and the other has two very small ports covered with micropore tape. I have been working on building plates the past few months, and finally got my B+ rhizo isolation where I want it. So, I am running two tubs: 1) From Tomentose (spell check) agar plate, original de-contaminated isolation (v1) 2: Rhizo agar plate start to Brown Rice, the agar was a 4th round of transfers and a beautiful plate (there are lots now ) pics below too.. Spawn: Brown Rice. I do not plan on running brown rice anymore, it is too prone to issues, over 40% of my jars went bad for one reason or another. I was particularly aseptic too, so I think it was really just a shit spawn issue. Sub: exotic poo poo mix from Poo God (ostrich, cow, some other bovine poo + some coir and additives I believe) The grain bag from tub "1" (tomentose LC bag) took forever, incredibly slow (like 6 weeks). The jar from my rhizo isolation plates took about a week and were beautifully mycelliated. I will post some images below and along the way! At time of writing, I am waiting for the tubs to be fully colonized.