Psilocybe phylogenetic tree with P. natalensis

Discussion in 'HUNTING & IDENTIFICATION' started by Alan Rockefeller, Oct 12, 2021.

  1. Alan Rockefeller

    Alan Rockefeller Moderator Moderator Expert Identifier

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    Someone sent me a Psilocybe natalensis ITS sequence a few months back, so I had a pretty good idea what P. natalensis was - but it wasn't my sequence so I didn't want to share it. This week I made a Psilocybe natalensis sequence in my lab, which matches the other P. natalensis sequence I have. I've also uploaded it to Genbank, the first sequence of this species there.

    The strain is Natal Super Strength.

    Photos of the collection: https://mushroomobserver.org/472561

    Here is a tree I made which includes P. natalensis and related species, with P. allenii as an outgroup.

    [​IMG]

    The Psilocybe cf. cubensis sequence is interesting - it's quite close to cubensis, and has some noise, but I don't think it's just a bad read of cubensis because it has some of the same nucleotide substitutions that Psilocybe ovoideocystidiata has. Gaston Guzman collected it in Nepal in October of 2001. Without studying the collection (it's in XAL) I can't tell if it's a new species or if it's something which hasn't been sequenced yet like Psilocybe pseudoaztecorum.

    Other things you can see in this tree are that Penis Envy has the same ITS sequence as regular cubensis, Psilocybe ovoideocystidiata from the east coast is the same as it is on the west coast and Psilocybe thaiaerugineomaculans is the same as Psilocybe thaiduplicatocystidiata.

    In case you'd like to experiment with making phylogenetic trees or analyze the natalensis sequence yourself, here's the FASTA file: http://images.mushroomobserver.org/natalensis.fas