Huatla de Jimenez Psilocybe caerulescens var. mazatecorum and Psilocybe zapotecorum

Discussion in 'ADVANCED MYCOLOGY' started by elfstone, Jan 20, 2013.

  1. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    Here are a few photos of the jars started from cultures collected by Azure in Juatla de Jimenez. The Ps. zapotecorum were collected on the path near Cerro Adoracion where the Ps. mexicana were collected. The Ps. caerulescens var. mazatecorum were collected from bagasse near where he was staying on the East side of town.

    caerulescens65.jpg caerulescens71.jpg caerulescens74.jpg caerulescens78.jpg

    The caerulescens is adapting well to the substrate, which was prepared as follows:

    4 cups ryegrass seed + 1 cup steel cut oats were soaked in water for 24 hours. This mixture was drained. 2 cups of brown rice was cooked for 10 minutes and drained. This was added to the ryegrass seed and oats mixture, with 4 tbs of gypsum. The mixture was spooned into quart jars (filled 4 quarts), then sterilized at 15 psi for 90 minutes. The jars were shook (it took about 50 taps to loosen them up due to the stickiness of brown rice; next time we'll try millet). Innoculated with agar wedges and shook again thereafter about every 3 days on 3 occasions over the next 10 days and then left to themselves.

    Here are the zapotecorum:

    zapotecorum66.jpg zapotecorum71.jpg zapotecorum75.jpg zapotecorum79.jpg

    They are being cased with a standard 50/50 casing mix, composed of:
    1 liter strained peat moss (to remove the chunks and sticks)
    1 liter vermiculite
    0.5 liter calci-sand
    1 liter distilled water
    Sterilize at 15 psi for 90 minutes

    Stay tuned...
     
    Last edited: Jan 20, 2013
  2. mycborg

    mycborg Well-Known Member Supporter

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    :popcorn:
    You sure got my attention!
     
  3. the_chosen_one

    the_chosen_one there are no answers.. only choices Moderator Mushroom Doctor

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    :thumbup1: subscribed.
     
  4. rogue

    rogue ♥ Hooked on Mycelium ♥ Moderator Mushroom Doctor Supporter

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  5. Professor PinHead

    Professor PinHead Lost in the Tek.... Administrator Mushroom Doctor Cannabis Doctor Supporter

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  6. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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  7. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    For those interested, my approach to casing is also fairly straightforward. I figure since I spent quite a bit of time assuring cleanliness to get to this point, I might as well be as mindful when applying the casing layer. I use black plastic garbage bags cut to fit the tray. I fit the bag such that the inside of the bag, which is sterile, is what will hold the substrate. I also have it cut long enough so that I can lay the other side over the top of the casing layer to keep in humidity while it colonizes. This allows me to avoid spraying it until the mycelium has fully colonized the casing layer. I use distilled water for everything as it is also bottled and sterile. The black plastic stops any primordia from forming on the sides of the culture and assures the pinning happens at the top.

    This is illustrated as follows:

    casing187.jpg casing188.jpg casing189.jpg

    I place the cultures in my humidity chamber. The humidity chamber is made from a 32L Really Useful Box that I picked up from Office Depot:

    www.reallyusefulproducts.co.uk/usa/html/onlineshop/rub/b32_0litreUS.php

    The humidity source is an Imagine Gold Tropic Aire Humidifier Air Exchanger:

    www.amazon.com/Imagine-Gold-Tropicaire-Humidifier-Exchanger/dp/B000634980/ref=cm_cr_pr_pb_t

    Which is powered by a Rena Air 300 air pump:

    www.amazon.com/RENA-AIR-300-PUMP/dp...?s=pet-supplies&ie=UTF8&qid=1331486285&sr=1-1

    Using a 15/64 inch bit, I drilled a single hole near the top, which lines up with the hole already present in the snap handle and lid. This size hole perfectly allows the plastic airline tubing to fit securely and snugly into the box.

    This is a shot of the total setup:

    fruitingchamber110.jpg

    And, it will be illumined to activate fruiting with a Truelumen LED Actinic Blue 453 light strip attached to the inside of the cover:

    actinicblue453-2.jpg
     
    mycomattie and MeriwetherLewis like this.
  8. Moondrake

    Moondrake New Member

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    Beautiful setup ES! I wonder if you use the blue LED strip only for pin initiation? Does this work with all the popular and exotic species?
    Also, the pump/air exchanger is very nice. Does this take care of all humidity and FAE? Seems like this system is just set up and sit back eh?
     
    Last edited: Jan 20, 2013
  9. Moondrake

    Moondrake New Member

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    So am I correct in believing this particular design needs little or no maintence throughout the pinning/fruiting process until harvest?
     
  10. mycborg

    mycborg Well-Known Member Supporter

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    Nice setup elf :super: I've been looking at that humidifier for a while now and if I run into a FAE problem(I probably will) I'm buying one of these. How much nois does that air pump put out? An air pump was one thing holding me back buying this.
    Some questions, sterile destiled bottled water?? Where do you get this? And how is it sterilized? Also you use sterile black garbage bags. How do you get this sterile? I assume it won't hold up in a PC?
     
  11. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    The distilled water is from a local bottled water company and is steam distilled. It has to be sold to the public already sterilized , All plastic garbage bags are already sterile inside due to the nature of the manufacturing process. I do not run it through any sterilization process.
     
  12. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    Elfstone, why do you sterilize your casing? Isn't the pasteurization the better thing to do?




    This is soooo true!

    I started to use distilled water for humidification and I must admit that the mycelium is behaving much better. Who knows what they put in tap water. We only know about chlorine.
    I alaways buy distilled and sterile water in pharmacy and use it for my syringes, because sterilizing the distilled water is unnecessary when you can buy sterilized.
    Sterilized water is used for syringes when they mix up a medicine coctail and it is available in pharmacies.
     
  13. mycborg

    mycborg Well-Known Member Supporter

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    Ah thanks Hacen. I have to check with my local pharmacy if they supply such thing here, it won't surprise me if not.
     
  14. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    For public safety, bottled distilled water is already sterile, though I wouldn't use it for purposes of making a sterile syringe just on principle that I want to be 100% sure it is sterile and so run it through a PC at 15 PSI if I plan on using it to make spore syringes.

    I sterilize my casing as I find I get good pin set and 4-5 flushes if I do so. Pasteurization, I find, takes more messing around with thermometers and such than I care to spend time on. When I have attempted pasteurization, the vector of contamination seems less predictable. I get consistent, repeatable results with simple sterilization. I also do relatively small grows and still find it produces way more than can be consumed.
     
  15. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    This is the sterile water I was talking about....

    DSC01487.resized.jpg
    Thank you for the info on distilled water :super:
     
  16. Trout

    Trout Closet Mycologist, Literaly! Mushroom Doctor

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    Lol. I just cased some caeru, zapotecorum and pseudoaztecorum. Two days later I'm looking at them to see if anything is happening, yeah right.
     
  17. rogue

    rogue ♥ Hooked on Mycelium ♥ Moderator Mushroom Doctor Supporter

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    what else you gona do :) :stare-at-sub: :clock-tic-toc:
     
  18. elfstone

    elfstone Initiate Mushroom Doctor Supporter

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    The caerulescens has peeked through the top of the casing, though not the zapotecorum. I'm told that patience is key, as in a month or so of patience....
     
  19. Trout

    Trout Closet Mycologist, Literaly! Mushroom Doctor

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    Okay I am going to say this and I certainly hope I am right about this. I have two bags of P. zapo. cased on 1/30/13 that are pinning in the bag. I have been disappointed before by weed fungus but I don't think they both would be contaminated. I also have P. pseudoaztecorum cased on the same day and it is fully colonized and reaching out, it looks like happy cube myc. I am just waiting for knots to form. It is in a fc exposed to fruiting conditions. Two caeru jars are also peaking through there casing from the same batch. Here are some bad pics through the bag. And one of the pseudoaz. image.jpg image.jpg

    If these turn out I will share the casing formula. All are ms grain spawn to a sawdust based compost mix. All were originally spawned on December 15, 2012.
     
    Last edited: Feb 14, 2013
  20. Hacendado

    Hacendado Family member Mushroom Doctor Supporter

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    Are fruiting them straight from bags or do you put the blocks in the gh?

    I am asking this cause I have villareliale and zapo blocks colonizing and I was wondering if I could case them and fruit them straight from the bags?

    If so, how often do you spray/aerate them?