Discussion in 'ADVANCED MYCOLOGY' started by elfstone, Jan 20, 2013.
That's a good excuse as any to head for the border..
Well, here we are, 6 years later and now we have clean spore prints of derrumbes collected in Huatla de Jimenez and more sophisticated technology at hand. Using a 6’ x 6’ x 8’ Homebox Growroom tent as a clean room with a Holmes Aer1 99.99% HEPA set in the corner which runs 24/7 to maintain clean air at all times and a Laminar flow hood sitting on a Sunco Stainless Steel work bench we have clean derrumbe mycelium, liquid cultured and poured into a Unicorn T3 mycobags, colonizing it in under a week.
Some of the cultures sector into thicker areas
Liquid culture is definitely the way to go:
And the 3:2:1 whole oats: rye grain: ryegrass seed supplemented with oat bran mycobag is colonized using that blend in under a week:
Next step, casing. Further updates to follow...
Awesome , I wish you the best with this. Will be following for sure.
looks badass so far
Pulling up a seat.
Just found this log. So much love. It's great!
I will be watching and wishing you more success!
So we’re now about to enter in to the fruiting stage. The derrumbes are in the far left, far right back and in the box in the front left. In the middle left are Jalisco and middle right is CNM. The bag on the HEPA is zapotecorum which was just cased, it has a “wonderbubble” waiting for it when it is ready to go into the fruiting stage (you can get them from Walmart for $13). They hold a mycobag and serve as a good fruiting chamber with an aquarium stone run into a layer of water/vermiculite at the bottom of the container. I use a rubber band and some Saran Wrap to secure the tubing and keep the humidity in and bugs out. Casing the mycobag directly is quicker and easier than messing with trays.
The substrate for the derrumbes is 3:2:1 whole oats:rye grain: oat bran. I added alder wood to the zapotecorum and 5% sand. The substrate for the CNM is 3:2:1 whole oats: rye: ryegrass seed. The Jalisco I put on straight whole oats to see what happens. I use LC and innoculate each bag with about 500ml - they fully colonize within 10 to 14 days that way. The fluorescent grow light has one 420nm veg blue light in it and 3 6500k lights. I also have a few other blue LEDs to trigger primordia formation.
So, for experiment No. 1 we shall see what happens.
So one of these wonderbubble holds a standard unicorn mycobag:
The casing is 10:1:.05 peat:calcium carbonate:sand
Here are the Jalisco:
And here are the caerulescens from Huatla
An update regarding the zapotecorum and caerulescens - although the substrate colonization phase is easy and swift using liquid culture techniques, the main problem that pops up is using pasteurized casing. These species take about 3 months to fully colonize the casing. The casing has been uncontaminated, but the peat moss itself starts to sprout after about 2 months greening up beautifully under the 420nm lighting. The caerulscens is now fruiting up through that casing, while the zapotecorum is just sitting there. I have decided to utilize a modified pftek method, using the mycobags, and will case with a straight up sterilized vermiculate/sand mix. The bag was made using 6 cups Espoma vermiculate, 3 cups of water, and slightly less than ½ cup of white sand and a tbs of gypsum. After mixing the vermiculate/sand/gypsum/water together, I then added 1 cup of brown rice flour, 1 cup of millet flour and 1 cup of whole oats flour. This fit nicely in the bottom of a mycobag which was clamped and sterilized for 1 hour at 15 psi. I then inoculated it with 600ml of a thick zapotecorum liquid culture, which was poured directly into the bag in front of the HEPA filter air stream. Once fully colonized, I will case with a vermiculite:water(2:1) casing with 5% white sand added in to the mix. (Casing mix: 2 cups of Espoma vermiculte, 1 cup water and 5% white sand (about 2 Tbs. sand).
My theory is that the mycelium rapidly utilizes the nutrients and exhausts the substrate and then fruits more quickly. With a sterilized casing contamination will not become a problem long term. The last time I used this approach I was successful getting the zapotecorum to fruit at about 2 months out from the initial spore inoculation on a petri dish. We will keep you all posted!
Look forward to hearing how it goes thanks for sharing
Got tired of the cultures taking up space in the lab and moved everything to a fruiting tent. This has a standard plant shelf, Hydrofarm Agrogro 24” T5 lights, two of which in each fixture are Eye Hortilux 420nm blue lights, a space heater and a setup with air stones for 4 Wonder Bubbles.
This keeps the lab ready for other culture work, such as growing Stropharia rugusoannulata and other species featured in Paul Stamets books. Fun fungi!
Yesterday I cleaned up some old plates of the derrumbes. They had some sclerotia-like structures that I decided to consume, thinking it was only going to be a “micro dose.” They completely swept me away. They are very strong! So, we have decided to experiment with growing the mycelium on brown rice, drying and encapsulating it as a quicker and more efficient method of working with them than trying to get them to fruit, though we are not giving up on the latter project. It took us a little time to get a good strain of the CNM going. I now have both zapotecorum and caerulescens cultures that form mushrooms directly off of agar. These are the ones we will be focusing our efforts on.
As noted in the previous post, our work continues on finding the optimal substrate for fruiting the Huatla species. One of the cultures formed fruiting bodies on agar and has become the focus of our efforts toward achieving a readily fruiting strain adapted to laboratory conditions. While cleaning up the old plates of Ps. caerulescens mycelium a few sclerotia like structures were noted to have formed on the agar and were consumed. The total amount was about a chunk the size of the last phalanx of a thumb. This resulted in a profound opening. This species has a resonance and a power unique to it. Given that this was the effect from just a bit of the mycelium, we have decided to focus another line of effort on growing the mycelium out on brown rice, dehydrating and encapsulating it for medicinal consumption. This would allow for rapid production of greater biomass for ready consumption, bypassing the slowness and difficulties of producing the carpophores. Folks are doing this for other medicinal species, such as Lion's Mane, Reishi, etc., and it seems a logical extension to turn our efforts in this direction.
Paul Stamets has recommended combining Lion's Mane, Psilocybe and niacin into a neuro-genesis promoting supplement. This could prove to be an ideal species for such use.
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