Frozen

Discussion in 'MUSHROOM CULTIVATION' started by Potato, Jan 6, 2014.

  1. Potato

    Potato Member

    Joined:
    Oct 20, 2013
    Messages:
    44
    Gender:
    Male
    I got packages today. One of the two syringes was froze. And the premade substrates were cold to touch.

    I'll hold off on using the frozen syringe until I have more materials to work with and not mind failing.

    If anyone has any knowledge or opinions about this predicament let me know. Please and thank you.
     
  2. Trout

    Trout Closet Mycologist, Literaly! Mushroom Doctor

    Joined:
    Dec 6, 2011
    Messages:
    2,400
    Gender:
    Male
    Location:
    My Garage
    Cold subs...just let then sit till room temp. Frozen syringe may be fine unless it was a live culture. But maybe not.
     
    Megan likes this.
  3. Potato

    Potato Member

    Joined:
    Oct 20, 2013
    Messages:
    44
    Gender:
    Male
    It was just a malabar spore syringe from the works. It thawed out. There was just a huge clod of frozen spores. The pe uncut ms wasn't as dark but wasn't frozen either.
     
  4. rabbit88

    rabbit88 Mad Scientist

    Joined:
    Oct 10, 2013
    Messages:
    321
    Gender:
    Male
    Location:
    Were The Wild Things Are
    Id say ure fine from wat I understand spores are pretty tough they can survive the cold and vacum of space I think a couple cold winter days arent gonna hurt the only problem I think there might be wen the h20 and air condensed it pulled in some contam with it even then id chance
     
    seeker likes this.
  5. Megan

    Megan Well-Known Member Supporter

    Joined:
    Oct 1, 2013
    Messages:
    428
    Location:
    The Bluff
    If I were you, I'd start the pe uncut, and use the malabar as an experiment to see how much of a beating spores can take
     
  6. Potato

    Potato Member

    Joined:
    Oct 20, 2013
    Messages:
    44
    Gender:
    Male
    I used mostly the peU for the inoculations. 2 projects: a brf jar and a brf bag were done with the one that had frozen. They are all incubating at around 76*-80* F. I labeled them and guess I will know something in a few days.

    I honestly think they're fine according to the information Rabbit stated above which I know I've read somewhere at sometime(the space spores theory and all).There is a spore site that sells freezable solutions. So most likely spores can withstand below freezing temperatures.
     
  7. nomendubium

    nomendubium scraping by, since '97 Expert Identifier

    Joined:
    Jan 18, 2012
    Messages:
    2,949
    Gender:
    Male
    Location:
    Ohio
    I looked at some Paeaolus cinctulus spores under the microscope a couple Weeks ago. They were left in my storage unit last winter.... Many of the spores were split open (and obviously ruined) some were not busted however so maybe it would still be viable. When water freezes to ice, it has more than enough strength to break the cell walls of spores
     
    cue likes this.
  8. rogue

    rogue ♥ Hooked on Mycelium ♥ Moderator Mushroom Doctor Supporter

    Joined:
    Jul 15, 2011
    Messages:
    7,032
    Location:
    Jefferson State, Hardiness Zone 7B
    I had a few spore syringes (cubes) freeze some time back and they were toast. Nothing ever germinated after that.
    If it were a print it probably would be fine but suspended in liquid seems to be a bit more brutal.
     
  9. Potato

    Potato Member

    Joined:
    Oct 20, 2013
    Messages:
    44
    Gender:
    Male
    I talked with spore works. They claimed unless they cracked from the ice expansion they'd be fine. It's been two days I'll wait a few more to see if anything takes.
     
  10. Forrester

    Forrester Active Member

    Joined:
    May 12, 2013
    Messages:
    576
    Location:
    Maine
    redcat likes this.
  11. seeker

    seeker New Member

    Joined:
    Jan 26, 2019
    Messages:
    5
    Gender:
    Male
    thanks folks i have some pe,s that i'm concerned with but i'll give 'em a whirl and see what happens
     
  12. seeker

    seeker New Member

    Joined:
    Jan 26, 2019
    Messages:
    5
    Gender:
    Male
    those pe's must not have been froze too long, mycelium going just fine. supprised and thankful, hopefully the rest will be smooth sailing
     
  13. seeker

    seeker New Member

    Joined:
    Jan 26, 2019
    Messages:
    5
    Gender:
    Male
    those pe's must not have been froze too long, mycelium going just fine. supprised and thankful, hopefully the rest will be smooth sailing.