injectable agar tek

Discussion in 'MYCO-TEKS' started by mellowmarsh, Jan 25, 2015.

  1. mellowmarsh

    mellowmarsh Member

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    This is not my tek, I seen it somewhere and have modified it the way I like. I started using it in 09 and it works very well.
    I use malt yeast agar and I use the 4 ounce ball mason jars my lid tek is to flip the lid over so the rubber seal faces up, and that's it the metal and glass will not seal so it gets gas exchange. I also only tighten finger tight. Not loose though IMG_20150113_190924831.jpg

    Inoculated with your favorite spores or isolated mycelium or whatever.
    Let colonize fully
    IMG_20150113_015716401.jpg
    Next take a sterile syringe with a 16 g needle 18 will work just not that good. Flame sterilize the needle and use it to scrape the mycelium of the agar. You want to make sure that you scrape it so your pieces of mycelium are small enough to fit in the needle. Looks like this. IMG_20150113_015935196.jpg
    Next take sterilied water, I do two full syringes if you do 3 there weaker but work.
    Add the water, put the lid on and swirl not to hard because there's no seal..
    Suck back into the syringe and use like a bad ass LC. IMG_20150113_244305673.jpg
     

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  2. SillyBear

    SillyBear Active Member

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    I have done this before exactly like you said works great
     
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  3. Papa_Legba

    Papa_Legba Member

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    :sporedrop:Nice
     
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  4. bobjonez

    bobjonez Member

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    Sup, mellow love this Tek!!!
     
  5. kingpsilo

    kingpsilo Well-Known Member

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    Ive thought about doing this but, slightly different.. Because I don't have a flow hood and can't open an agar jar out in the open, I made an injection port on the top of the agar lid.. I figured once the mycelium is fully colonized, id just shoot in some sterile water and swirl it around and suck up what I can then innoculate a new jar of LC with it.. Then It'll grow in there and will give you many more inoculations.. Might not be as potent as your method, but I believe after it colonizing for a while, you'll be able to draw up a lot more myc. in each load.
     
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  6. cue

    cue Well-Known Member

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  7. rogue

    rogue ♥ Hooked on Mycelium ♥ Moderator Mushroom Doctor Supporter

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    I feel more comfortable using a filter on my lids. :shrug: They are very cheap and provide a level of peace of mind. IMO

    Air is going to flow in the path of least resistances, I prefer to "control" the path of air exchange/gas exchange through a "known" filter size, rather than the very loose tolerances of a loose fitting lid.

    It just may be that a sealed jar has plenty of air to provide good colonization, so that is even better than a filter. My only concern then would be for slower growing species and possible negative pressure when opening to inoculate.

    That's my $0.02
     
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  8. Rockstarr

    Rockstarr New Member

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    Does the mycilium grow back since you only scraped it?
     
  9. Rockstarr

    Rockstarr New Member

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    August 2015? Wow, im out in the boonies
     
  10. SleepDeprivedad

    SleepDeprivedad Active Member

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    Wow never thought of this can you use this in the same sense as when you transfer a nice section to your last agar and just throw it in there instead? I would assume @Rockstarr if you leave a nice little bit on the agar it would grow back right?