Down n Dirty Breeding

Discussion in 'ADVANCED MYCOLOGY' started by ivangrozny, Sep 24, 2015.

  1. ivangrozny

    ivangrozny Active Member

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    OK, so over the years you run into a print that just wont fruit, everything seems normal about it but it seems really resistant to fruiting. I got thinking about this. Could it be that a mechanism to insure genetic diversity was responsible for this? Maybe. Maybe in a wild or near wild strain this could be prevelent in some lines. In a highly selected line maybe we have reached a point of inbreeding depression. Never heard of this discussed, but nature really dislikes things that lack genetic diversity. So what can we do to save the genetics, and below is something I hope to experiment with and see what happens.

    Now I could follow the accepted method of growing identifying out monosporal isolates, but god damn what a pia that is. So down and dirty breeding, streaking from 2 different prints on the same plate, or I think what may work better is to mix them together in a small quantity of water and then streak to agar. Ideally this would be a small amount of each prints spores, diluted to maximize randomized matings. Will it work, no clue, but it makes sense, at least to me.
     
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  2. RhizoDork

    RhizoDork Fake it till you make it

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    I'm in the front row! Are you going to do two domesticated strains? Or a domesticated and a wild to add the diversity you're looking for?
     
  3. ivangrozny

    ivangrozny Active Member

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    I think both would work. But I don't have any wild cube prints to play with so they will be domesticated strains. Maybe I can sucker you into trying this as well. Results wouldn't be conclusive mind you, but I am ok with empirical evidence.
     
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  4. RhizoDork

    RhizoDork Fake it till you make it

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    I'm down! Maybe if I get this wild one to fruit, I can use a clean, very wild, print. Fingers crossed. If not I have BM, that's still pretty wild as well. I can send you one if you want.
     
  5. ivangrozny

    ivangrozny Active Member

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    cool, I'm down for that
     
  6. RhizoDork

    RhizoDork Fake it till you make it

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    So what do you think would be better to put the diluted spore solution onto a plate with Ivan? Loop or sterile swab?
     
  7. ivangrozny

    ivangrozny Active Member

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    I prefer a loop since they can be flamed between uses, but a swab works.
     
  8. RhizoDork

    RhizoDork Fake it till you make it

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    K. Let me know when you get your correspondence. I'm going to make more agar plates this weekend.
     
  9. Titus

    Titus Creative PITA

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    I prefer a loop as well, hobby knife has worked for me as well..
     
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  10. RhizoDork

    RhizoDork Fake it till you make it

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    I was referring to getting the spore solution streaked onto the plate. Will a loop do that well?
    I dunno, I just got one, so I'm not real savvy..
     
  11. ivangrozny

    ivangrozny Active Member

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    yup, it works great
     
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  12. Titus

    Titus Creative PITA

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    You can also use a loop to blow tiny bubbles.
     
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  13. RhizoDork

    RhizoDork Fake it till you make it

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    You're a fucking goofball, Titus.
    got time.jpg
    :)
     
  14. ivangrozny

    ivangrozny Active Member

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    Thanks Rhizo, it's on mofo
     
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  15. RhizoDork

    RhizoDork Fake it till you make it

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    It's on! I think you know what you got there. Any
    questions, just ask. I'm gonna use AA+ with BM rust...I'll let you know when I get some plates together this weekend.
     
  16. RhizoDork

    RhizoDork Fake it till you make it

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    So just for the hell of it, I mixed up some AA+ spores with some Blue Magnolia Rust, swabbed two plates with the solution. We'll see...
     
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  17. kingpsilo

    kingpsilo Well-Known Member

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    I did this with the gt's and ksss and got some sweet lookin fruits.. Def noticed traits from both strains..
     
    Last edited: Jan 2, 2016
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  18. RhizoDork

    RhizoDork Fake it till you make it

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    This was a fail. I'm not sure what happened, bad agar work probably. I got some plates in jars, and they greened up on me. Blah. Might try it again one day in a different way. Maybe straight into grain.
     
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