Zero to No Growth in 1/2 Pint Jars.

Discussion in 'MUSHROOM CULTIVATION' started by wilspeak, Nov 3, 2017.

  1. wilspeak

    wilspeak Active Member

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    I am new to (PF BRF/VERM Tek) There is hardly any growth in jars 1,2 and 3. Jar 4 shows No growth. This is going on 3 weeks . I followed some advice and turned the jars over that helped a little buy not much. I opened one of my jars (#4) that showed no growth and it didnt have a bad smell , it smelled earthy also the verm had the consistency of play-doo clay , is it too dry? What is wrong and what should I do the next time I make new jars ?
    Thank You

    [​IMG] Screenshot (29).png
     
  2. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    Did your spores come from a reputable vendor?
    You don’t have to name the source ,
    But i would think a week or so is a decent time to see germination at room temps
    2 weeks is plenty long enough
    So I’d say 21 days is bad spores
    From the pic , I see something germinated on jar 1
    Best thing to do is don’t look at them at all
    Turning them upside down and opening it is not good advice.
    At this point ,probably start some new ones
    Not saying you would never get colonization , but one brf cake is going to take the same effort as 12
    Good luck , be patient
     
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  3. wilspeak

    wilspeak Active Member

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    Hello PE-n-hed, That one jar , Jar#1 is growing a little bit, but I am doubting that it will get to 100% at this rate , But I am going to let sit for a month or so and grow IF it grows. As for the rest I am thinking about trashing those. I already threw out jar#4 and when I opened it was like clay and didnt smell bad. there is a layer of verm on top
    2- I am trying to grow A+ Albino
    3-I looked to see signs of contamination and could not find any. And I I followed the BRF and Verm water recipe (2.1.1 ... FOR EVERY 2 CUPS OF VERM ADD 1 CUP OF BRF AND 1 CUP OF WATER). I ordered the spore syringe from spore works. I am going to try again. This time I think the next batch I make i will do a mix of BRF/VERM jars and a batch of agar and grain, and see what happens.
     
  4. cue

    cue Well-Known Member

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    What temp are they being stored at?
    IMO 70F is OK, 75F is better, but I prefer 80F.

    Side note, your verm looks finer that I liked using.
     
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  5. wilspeak

    wilspeak Active Member

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    Hello cue, If you look at Jar#1 up top , ITS GROWING :apple:, not 20171108_220740.jpg much but its bigger than when I took that picture last week. I just wonder IF and WHEN it will get to 100%, its already been since 10/27/17.
     
    Last edited: Nov 9, 2017
  6. FewTrueSeed

    FewTrueSeed Many meiosis Supporter

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    Thats a long time. What is your method. Injection port? How did you sterilize? Calculate moisture content? Im inclined to say that bacteria is your culprit. Walk us through it and we can diangnose and try again. :)
     
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  7. wilspeak

    wilspeak Active Member

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    Hello, I would first like to say I inoculated the 4 jars 10/27/17. It looks like only one is growing. This is my first grow. I tried to follow all the sterilization procedures. I made 3 holes in the lid..... the air hole in the middle which I first used poly fill , but then I changed it to miro tape and turned the jars upside down for a couple of days ..., THATS WHEN THAT ONE JAR STARTED TO GROW. The other 2 holes are the inoculation holes filled with silicone . Only one jar seems to be growing the others are at ZERO OR NEAR ZERO GROW. Thank You
     
  8. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    dry verm layer on top?
    If you have the verm layer on top, no need for the poly or micro pore tape
    Or even the silicone....
    That lid set up is better suited to grain
     
    Last edited: Nov 9, 2017
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  9. wilspeak

    wilspeak Active Member

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    Hello PE-n-hed, The reason why I put Micro tape on it is because I was afraid of contaminations. But if you think it would be better I can remove the tape. What do you mean by >>> That lid set up is better suited to grain ? I have BRF and Verm, is Grain better ?
     
  10. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    I haven’t made a brf cake in like 9 yrs, but if I did .... the lid would just have 4 holes big enough for the needle . 1/4 cc per hole = 1 cc per jar
    More inoculation points = more germination points = faster colonization
    I’d get a case of short wide 1/2 pint ball jars and try to get all 12 out of 1 syringe
    Bout a 1/2 inch layer of dry verm for the filter
    Set on shelf and take a look after a week at one jar
    Don’t tilt , look , shake , jostle , flip the jar till it’s done
    After 100% wait another week
     
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  11. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    Nothing wrong with brf cakes
    You can use them as spawn for bulk sub as well
    Just depends on your needs
    Yes grain is a better route to more spawn via g2g
    I don’t recommend spores to grain.... but it can work
    Agar 2 grain ,then g2g works well
    Don’t rush in, grow a few cakes to learn with
     
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  12. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    Do you have a pressure cooker yet?
     
  13. wilspeak

    wilspeak Active Member

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    First off I am a "Newbe" My first BRF jars were on 10/27/17, thats why I am asking a lot of questions. I only put 2 inoculation holes in the lids, because I thought 4 would be more openings for contamination, but thinking about it ...... now I should have put 4 holes in the lids. The next batch of BFR jars lids will have 4 inoculation holes. I have never tried Grain 2 Grain . I do have a old PC , But I didnt use it for my first jars because I was afraid of burning the jars up because my PC does not have a gauge.. I used a large pot and boiled the jars for 2 and 1/2 hrs. I sterilized 4 jars , only 1 looks like its growing and that one is growing slowly. The next time I am going to use my PC for Agar . and the BRF jars. The only thing my boiling pot is bigger and can do more , and the PC is small and can only do 1 or 2 jars at a time.
     
  14. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    Steaming brf works
    That’s why it’s a starting point
    If your serious about the hobby, you’ll end up buying yourself a nice PC
    You have growth.... you did something right
     
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  15. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    Ask all the questions you like
    That’s what this site is about .
    I have been busy with life , and not very active lately .... but always glad to help out
     
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  16. wilspeak

    wilspeak Active Member

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    Thank You I appreciate your time and help , I am going to be perparing to make some agar petri dishes and then inoculate them. What are the next steps after the agar dishes are fully grown? Thank You
     
  17. PE-n-hed

    PE-n-hed Well-Known Member Moderator Mushroom Doctor Cannabis Doctor Supporter

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    We can cover all that soon enough
    With out a flow hood , no pour agar is way easy
    I have a hood and still used them
    Never used a single petri
    Find prof no pour tek
     
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  18. wilspeak

    wilspeak Active Member

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    I will look up no pour tek . I just made a Still Air Box today >>>>
     
  19. MadChatter

    MadChatter Member

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    SABs are really good, I literally get no contams on my plates (after my first 100 'tammed plates). The trick is I rinse my ketchup cups with peroxide and a bit of isopropyl alcohol before pouring my agar into them. Then I let the peroxide evap/condensate from my plates(1 or 2 days), before I innoculate, so that the spores will not get burned. I also work on Liquid Culture inside the SAB (unmodified lid). I just unscrew the lid a bit, once or twice a day for gas exchange. Works well, no contams :b1:
    Good luck on your adventure!