Need help fighting contams

Discussion in 'CONTAMINATION' started by Bobby Spoto, Nov 8, 2017.

  1. Bobby Spoto

    Bobby Spoto Member

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    Contamination is definitely a bitch it has been ruining everything I have worked my ass off for. I have spent so much money and time the past 4 months on mycology and I have gotten a total of a few brf cake flushes and two mushrooms in a 5×5×5 plastic container of alacabenzi and 4 mushrooms after about 5 weeks in a 16 qrt mini mono PE substrate. I'm hoping I can credit all of this 2 contamination and being a newbie as I thought I've been doing everything by the book. I will put some pictures up of two mini monos that I have, golden teacher and also for the record just recently as in last week I just made my first substrate and used a coir / verm / pickling lime / gypsum / manure and I tested it out with two 4×4×4 little mini containers but they're coming along a bit slow but that was just a test all my other substrates have been ordered from shroom Supply. So I put my golden teacher mini Monos and one ALAC in a small fruiting chamber with perlite in the window and started to look like it was contaminating big yellow oily spots and so I cut it out replaced it with substrate I also filled in the sides with substrate because it was making an open area and I did not want side pins so I filled the sides with substrate and seems to be contaminated now because nothing is happening whatsoever it's just getting more yellow and even the little Alac that's in the center as you'll see in the pictures which I didn't touch at all other than mist and fan, that's not doing anything and today is day 17 for the 2 mini monos and the little one is alacabenzi day 14. Still have three small trays of penis envy and two medium size trays of Alac and the two little trays that I mentioned with my new substrate that I made that's also Alac so I'm going to probably throw those in my Martha or another small fruiting chamber anybody have any input on what I should do and how I should do it? I can keep my temperatures low in the house and I use natural light for my lighting because I can and I can keep the temperature controlled pretty much so what else am I missing please help I want to see a flush I'm not going to stop until I do I've been at this for for 5 months now and about a grand into it and I still have not gotten one single flush other than the brf cakes. Have 5 spore syringes coming and a bunch of grain 20171108_131458.jpg jars made up ready to go to give this one more try with the knowledge that I've been gaining from trial-and-error the past 5 months anybody have any input please
     

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  2. Vegeta91

    Vegeta91 Member

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    Walk us through your process for inoculation, spawning, and transfers - what equipment are you using? What safeguards are in place to prevent contamination? Is everything being done in a sterile room? Make sure you decontaminate the room and your immediate area before starting anything with lots of Lysol and alcohol.

    When I prep my lab, I'll often use up to 1/4 of a bottle of iso alcohol just to clean and re-clean every contact surface in the room. Full contact precautions on the operator's end as well - hood, goggles, N99 mask, gown, sterile gloves - rinse them w/ alcohol EVERY time you touch something - for example, put down a jar of grain spawn, rinse w/ alc, pick up bag & open in front of laminar flow hood, rinse again, transfer grain from jar to bag & set jar down, rinse again, seal bag, rinse, go on to next project. If something is contaminated, I just toss it immediately. It's nearly impossible to kill off contaminants once they take hold. It's far better to lose one tray of bulk sub than to contaminate the entire lab.

    Another thought, molds and bacteria LOVE carpeting -it's dark and holds moisture, and you're constantly adding more little nasties every time you walk on it. Consider steam cleaning the carpet in there to kill everything off - it won't prevent all your contamination issues, but it doesn't hurt to keep everything as close to sterile as possible.
     
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  3. Bobby Spoto

    Bobby Spoto Member

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    Thank you for that info you're making plenty of sense my sterilization techniques can definitely be improved and I do have Shaggy carpet in that room and I know that's probably infested with contamination which is why I do a lot of cleaning as well I keep Lysol and bleach and alcohol everywhere with Rags I have gloves but I've never gone to the extent that you were talking about I also have my hand sanitizer right there a big bottle i squirt on my hands when I do my grain to grains or spore innoculation every time so I know practicing sterile techniques is a big factor in being successful so thank you very much for the revised version anything else you can add or say regarding my pics will be helpful. And I'm also setting up my Martha which I have a thread posted on the myco tek or cultivation and I just sterilized it and painted it with rust sealant paint also made more holes and cover them with micropore and I have a thread asking for any input on pretty much a self sustaining grow tent. I have a timer I have cool mist humidifiers I have natural lighting and I just ordered 3 CFL bulbs just in case I decide to go with that style of light but in the last five months I've learned a lot and I have 5 Spore syringes on the way grains ready to go and I'm going to approach with a whole new angle which is why I have been doing as much research, reading and reaching out to as many people as possible so I don't screw this up again. I have at least a grand and about 5 months into this so I would like to see at least one full flush..thanks again for reading..
     
  4. Vegeta91

    Vegeta91 Member

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    All I can say regarding the pics is that shit is heavily contaminated, toss it and decon everything before you try any more. Did you pasteurize your sub before you tried spawning? You didn't say in your original post.

    Walk us through your process:
    -how are you inoculating your grain (spore injection, grain-to-grain transfer, liquid cultures, etc?)
    -Where are you inoculating it (open air, sterile glove box, oven door?)
    -Is everything being properly pressure cooked (15 psi, 45 mins) or autoclaved prior to inoculation?
    -How much spore solution (or grain/LC) are you using per jar/bag of grain?
    -How are you spawning to bulk sub? Walk me through your process, start to finish.

    Given the trouble you're having with bulk, I'd recommend starting with a couple BRF cakes and getting your sterile technique down before moving on to spawning bulk. It's cheaper and much easier, though it won't produce nearly as much as you can with bulk bins. Just a good way to learn the process.
     
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