Atl7 or yet more bad luck?

Discussion in 'CONTAMINATION' started by n0j0y, Jan 3, 2018.

  1. n0j0y

    n0j0y Active Member Supporter

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    If you've been following my first grow log you've seen me make 2 attempts with two Petri dishes each of atl7 to agar and both came out looking quite weird and I was told to discard. Assuming it was the syringe and that I happened to have a second I did another couple plates on the 22nd. This is what I came back to IMG_20180103_102425.jpg


    The far right one my syringe went silly and I did a large sploosh on the plate. Either way this looks a little more like what id expect except the coloration. Lots of browns like before. What are people's thoughts? IMG_20180103_102436.jpg
     
  2. eLShaMukO

    eLShaMukO Moderator Moderator Mushroom Doctor

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    looks like mycelium make a few transfers from plate number 1 and 2 , number 3 looks weird
     
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  3. n0j0y

    n0j0y Active Member Supporter

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    Will do, guess I shoulda thought to do that while I was working on a few other things today. Third one was about 1/4 cc spray when I got over zealous in my dropper action. But yeah does still look weird. Will see what comes of some transfers.

    Thanks for the response.
     
  4. rhizoRider

    rhizoRider Active Member

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  5. rhizoRider

    rhizoRider Active Member

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    You salvaged them just keep at new transfers and cleaning your culture
    :congrats: you did it.
    Good things usually don't come easy.
     
  6. n0j0y

    n0j0y Active Member Supporter

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    Eh haven't salvaged anything yet. Just have been trying new drops from spore syringes. Will see what I can do with this. Reminds me I need to cook up some more agar tomorrow.
     
  7. rhizoRider

    rhizoRider Active Member

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    Those are miles better than first plates your gravy, just in for the transfer game now.
    You have some nice sectors to start with too.
     
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  8. tedwilson40

    tedwilson40 New Member

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    yes, looks good! I once had an atl7 syringe and like a dumbass I held the plunger as i shook the hell out of it. the whole syringe flew apart and the spores flew everywhere!!! I quickly managed to jam it back in and had a couple of drops left to inoculate with. to my bewilderment no contams on any of the plates!?
     
  9. n0j0y

    n0j0y Active Member Supporter

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    Guess I hadn't updated this. Got some decent looking cultures got jars going, g2g those into 2 4quart filter bags and shelved. Will see how they do.
     
  10. tedwilson40

    tedwilson40 New Member

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    dont forget to throw in some shrapnel to encourage more sclerotia. they like to grow on stuff so the more surface area you give them to cling to the better.
     
  11. n0j0y

    n0j0y Active Member Supporter

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    Eh they are sealed and shelved. It'll be what it js
     
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  12. tedwilson40

    tedwilson40 New Member

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  13. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    What do you throw in your jars ?
     
  14. tedwilson40

    tedwilson40 New Member

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    bits of plastic (broken jar lids ect...). anything that wont be impossible to separate or clean off once the sclerotia are formed.