No gaskets on jar lids. Do I need to re-sterilize?

Discussion in 'CONTAMINATION' started by That_Idiot, Feb 5, 2018.

  1. That_Idiot

    That_Idiot Member Supporter

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    I purchased some of these lids. I prepared some dextrose liquid culture medium, slapped the lids on the jars, autoclaved the bunch, and then read the following on the website: "A gasket is recommended for use with this lid if a completely watertight seal is important."

    So my question is - do I need to buy gaskets, install them, and then re-sterilize everything? Or will I be OK as is.
     
  2. n0j0y

    n0j0y Active Member Supporter

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    I'll let others with more experience add their opinion, bit given a lot of folks that do metal lids with holes in them with sfds put the metal part upside down so the rubber doesn't seal, I would THINK those would be ok assuming they tighten down nicely still.

    The logic I've seen is the air exchange will go through the easiest flow which would be the filters.
     
  3. That_Idiot

    That_Idiot Member Supporter

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    That is what I was hoping to hear. BUT I will hold off until I hear more. Thanks for your input.
     
  4. n0j0y

    n0j0y Active Member Supporter

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    Yeah I'm still very noob just basing info off other things I've read but I cannot speak to the plastic jars.

    I would say when in doubt re cook though. Shame to risk waiting weeks to get contamination because you skipped 90 min more.
     
  5. That_Idiot

    That_Idiot Member Supporter

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    Any idea how long these will be "sterile" after they come out of the autoclave?
     
  6. n0j0y

    n0j0y Active Member Supporter

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    Hypothetically if they are truly sterilized and sealed they would stay that way. Of course it isn't truly either of those things so my personal preference is get what you want going in there asap to get it a head start. I believe I've seen some people putting sterilized but not innoculated jars in fridge to help keep longer, which makes sense since molds and what not tend to prefer warmer temps too.
     
  7. That_Idiot

    That_Idiot Member Supporter

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    so waiting for a few days might not be a good idea?
     
  8. n0j0y

    n0j0y Active Member Supporter

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    Pretty sure ive seen general consensus on the question that a few days is likely fine. Better in fridge just to be sure. The way I see it is unless utterly sterilized, its a race to colonize and I don't want my guy to start late
     
  9. That_Idiot

    That_Idiot Member Supporter

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    Yeah. I autoclaved them Saturday morning att 17.5psi for 40 minutes or so in an All American Electric Sterilizer. That should do it, I hope. I will not transfer to the fridge just for good measure.
     
  10. n0j0y

    n0j0y Active Member Supporter

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    I just reread the initial post and realized we are talking liquid culture. I was thinking we were talking grains. Likely same answers but just wanted to point out I was thinking grain in all of my discussion and have less background on lc.
     
  11. That_Idiot

    That_Idiot Member Supporter

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    Ah ha. Thank you. I wish we could get some supplementary input to this question, too.
     
  12. n0j0y

    n0j0y Active Member Supporter

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    Yeah they will likely chime in but some tend to only check in randomly. I know in general there does not seem to be much love for lc over all here.
     
  13. That_Idiot

    That_Idiot Member Supporter

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    Ah ha. Is agar the preferred method? I've got spore syringes I need to convert to something more expandable. I don't have a laminar flow setup, so I use a plastic box with sleeves and gloves. This is actually my first time using anything other than store bought spawn.
     
  14. n0j0y

    n0j0y Active Member Supporter

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    Yeah most here recommend agar. No pour method in teks seem to work well for folks to not have to pour the agar and innoculate in still air box.

    I'm playing around with lc right now just to try it but I'd always want to test it on agar to see of it's clean either way.
     
  15. That_Idiot

    That_Idiot Member Supporter

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    Thank you. I'll have to look into this no-pour tek.