Testing a room for contamination

Discussion in 'CONTAMINATION' started by T_sam87, Sep 5, 2018.

  1. T_sam87

    T_sam87 New Member

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    hey all,i recently battled a trich contamination unlike any ive dealt with in the past. I was forced to stop production and since have bleached washed every inch of the room, a couple times even, i figure im about ready to start up again but id love to somehow test and see if i was able to get the contamination down to a manageable level. Whats a good diy way to test the room for contamination? I was thinking i could leave a few petri dishes or grain jars open in various parts of the room for a day, close em up, remove from room, and then monitor how long it takes to show signs of contam. What would be an acceptable amount of time for it to go before showing signs of contam?

    anyone else have any other ideas?

    Thx
     
  2. LuckOfTheFryish

    LuckOfTheFryish Moderator Moderator Expert Identifier

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    Thats an interesting question. Ill try to dig into that and see what i can find out
     
  3. T_sam87

    T_sam87 New Member

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    Thatd b amazing! Obviously im trying to avoid anything non-DIY. i think an open petri dish/grain jar would do the trick, but then the question is how do you guage your level of contamination based off of how quickly/aggressively the dish/jar is taken over
     
  4. LuckOfTheFryish

    LuckOfTheFryish Moderator Moderator Expert Identifier

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    I think with an open dish, there are to many inconstant variables. Something will always find its way to an open dish. Even if its stray trich from an outside source
     
  5. T_sam87

    T_sam87 New Member

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    Good point
     
  6. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    Try starting with a new culture. Maybe your original culture has hidden contams. When are you having contamination problem ? Are you giving enough FAE ? It could be an FAE problem. I had a contamination problem not too long ago that turned out to be a flowhood problem. Could you give us more details of your process ?
     
  7. T_sam87

    T_sam87 New Member

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    I lost a large amount of monotubs at the same time. 30/30 all contaminated before finishing a first flush, most before even starting a first flush. I keep things pretty tidy and have a laminar flowhood, been doing this nearly a decade. My take is that I may have opened one too many contaminated tubs in there and over the last months the situation got out of control, add a window AC unit to the situation and it's my guess this changed the units primary function from ac to constant mold disperser. I've never seen so many tubs bite the dust at the same time. Happy to answer more questions about the process but honestly i keep things pretty standard and inline with information Ive gathered from this site
     
  8. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    Im sorry this happened to you. I thought i was the only person able to lose 30 tubs at once. Theres so many things that can go wrong. A few times ive accidentally left the AC unit on blowing air while doing flowhood work and also had exhaust hoses detach while doing flowhood work. I think i lost 100 quart jars at least twice. Ive found that when i lose everything it was because something was blowing while doing flowhood work. Thirty tubs...damn...that sucks.
     
  9. T_sam87

    T_sam87 New Member

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    Not an easy pill to swallow! I lost 100+ quart jars from working in the same room too. I tweaked all sorts of things and couldn't figure out the problem. I always work in still air. It seems the only consistent variable could be the state of the room
     
  10. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    How about the air conditioner ? Is that a new addition ?
     
  11. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    Do you wrap the top of your tubs with plastic wrap ?
     
  12. T_sam87

    T_sam87 New Member

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    Yes on the plastic wrap. The AC has been there for a few years. I'm an idiot and didn't realize how window units work so it's basically just been recirculating mold throughout the room is my guess. I've since bleach cleaned it and the filter

    I think the plastic wrap didn't even matter cuz the tub was assembled in the contaminated room
     
  13. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    I hope you've figured it out as an unclean AC. Those filters can get pretty nasty. You probably know but dont let the cold AC air shoot directly onto your tubs. I also get more contams when my jars go past G3,G4...just a thought. Build your tubs in front of the flow hood if you arent already. Good luck !!! I hate mystery contams.
     
  14. T_sam87

    T_sam87 New Member

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    Not sure why I haven't been doing tubs in front of the hood. I'll have to start doing this
     
  15. redcat

    redcat The Myco Machine Moderator Mushroom Doctor Supporter

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    You may want to put a wire screen over the front of hood to stop anything from bumping(and denting) the filter. When you start making tubs in front of it you may dent the filter like i did. The screen will prevent any filter damage. I just bought a screen for my hood and wish i put one on a long time ago. Fortunately theres no major damage yet.
     
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