Stalled cakes

Discussion in 'MUSHROOM CULTIVATION' started by Mainframe, Mar 5, 2019.

  1. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    Hey crew. As you know I've had a little success with two out of ten cakes. But what's going on with the others. They have been in fruiting for over a month now no pins, no contams. Should I dunk again? But more importantly what could be causing this? Temps at 75-80 degrees. Fanning misting etc...
     
  2. MycoFreaK

    MycoFreaK Member

    Joined:
    Dec 30, 2018
    Messages:
    81
    Gender:
    Male
    Location:
    Pittsburgh,Pa.
    Did you let the cakes consolidate long enough? Sometimes cakes just never fruit. Are u using a properly built SGFC? I have a hunch though, they weren't fully colonized and will take longer to fruit as full colonization of substrate is one of the key pinning triggers for cakes, and bulk substrates.
     
  3. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    I mean it's my first go at this but all cakes we're colonized and then I let them sit another week and more time for some. Some took a few extra weeks to colonize... But maybe your right. I've read that in plenty of instructionals
     
    MycoFreaK likes this.
  4. MycoFreaK

    MycoFreaK Member

    Joined:
    Dec 30, 2018
    Messages:
    81
    Gender:
    Male
    Location:
    Pittsburgh,Pa.
    Ok so they should have been consolidated after 7days. I hate to say it but for some unknown reason to me, IME some BRF Cakes just never end up pinning or fruiting. Just like some bulk subs never fruit, tho that is usually do to a contam of some sort. Go to bulk growing in monotubs.
     
    tazzman and Mainframe like this.
  5. MycoFreaK

    MycoFreaK Member

    Joined:
    Dec 30, 2018
    Messages:
    81
    Gender:
    Male
    Location:
    Pittsburgh,Pa.
    Learn bulk grain jars and working with agar plates. My advice to you.
     
    DreamingRaven and Mainframe like this.
  6. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    Yeah that's kinda what I was thinking... Bummer. But I've got 5 qts of grain I'll post a pic of later. I think it's almost ready for a tub. Got an extra qt of rye I'm gonna g2g into 6 qts of wbs.
     
    MycoFreaK likes this.
  7. MycoFreaK

    MycoFreaK Member

    Joined:
    Dec 30, 2018
    Messages:
    81
    Gender:
    Male
    Location:
    Pittsburgh,Pa.
    How did u inoculate ur grain? Agar, LC or Multi spore inoculation? Just curious.
     
    Mainframe likes this.
  8. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    Lc. Also expanded lc... And noccd up a bunch more grain with one of the lc I made and nothing is going on with those... Idk if it went bad before I used it because i went easy on the malt? But I expanded to 3 different sized jars and the qt of lc looks great in the fridge, but I'm afraid it won't work either ‍♂️. Hence the reason I'm about to try g2g.
     
    MycoFreaK likes this.
  9. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    I really need to try making some agar. I don't own a scale and the first time I made it, it was a little too clear looking. Idk I've got plates I guess I could try pouring in my glove box.
     
    MycoFreaK likes this.
  10. n0j0y

    n0j0y Well-Known Member

    Joined:
    Nov 19, 2017
    Messages:
    545
    Or try the no pour method. That way the only time you are exposing is just to inoc and in theory you could do injection ports.

    Personally I hated working in a cab and built a flowhood pretty early on in the hobby. I recommend it as it gives you a lot more freedom of what you can do.
     
    DreamingRaven, PE-n-hed and Mainframe like this.
  11. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    I'll build one as soon as I can afford. I'm behind on the mortgage so it may be a while lol.
     
  12. MycoFreaK

    MycoFreaK Member

    Joined:
    Dec 30, 2018
    Messages:
    81
    Gender:
    Male
    Location:
    Pittsburgh,Pa.
    I've been keeping my eye out for an affordable laminar flow hood. I can spend around 250$. They're harder to find surprisingly for under 600$ some are 2grand! I can't build my own, I'm horrible with construction, LoL!
    Right now I use a Still air box sanitized with a sqirt bottle of high percentage of bleach to kill everything inside the box. I do my agar work in there and grain inoculations as well as g2g transfers..
     
  13. n0j0y

    n0j0y Well-Known Member

    Joined:
    Nov 19, 2017
    Messages:
    545
    Was the first thing I ever really built. Can be done with a hand saw and a drill, some screws and some calking. The proper filters can be a bit pricey, I think I paid around 200 for mine but it's 24x24 so lots of room to work when working with bags.
     
    MycoFreaK likes this.
  14. MycoFreaK

    MycoFreaK Member

    Joined:
    Dec 30, 2018
    Messages:
    81
    Gender:
    Male
    Location:
    Pittsburgh,Pa.
    I'll be looking to buy a used one I think. Possibly build my own IDK
     
  15. n0j0y

    n0j0y Well-Known Member

    Joined:
    Nov 19, 2017
    Messages:
    545
    Id expect the size and weight to make them a beast to ship if buying used. Good luck with it, I just see this as a hobby, learning to build stuff was a side quest to make this one easier and more affordable.
     
  16. Ozzy

    Ozzy Moderator Moderator Mushroom Doctor Cannabis Doctor

    Joined:
    Aug 20, 2011
    Messages:
    1,418
    Gender:
    Male
    Location:
    In your bushes
    I am tipsy but I don't think you got your answer to the questions...if it looks dry dunk...fan and mist . more..if nothing happens then toss em it's prolly a bacteria k fectikn
     
    Mainframe likes this.
  17. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    Thanks!
     
  18. MycoFreaK

    MycoFreaK Member

    Joined:
    Dec 30, 2018
    Messages:
    81
    Gender:
    Male
    Location:
    Pittsburgh,Pa.
    *Agar is the best! I started out and didn't know how to make it so I ordered SABDEX Agar plates off Amazon.com.
    I've now got plates on their 2nd, 3rd, 4th and even one on a 5th transfer. I also have master plates stored in my mushroom supply only refrigerator. I like the agar with antibiotics in them like chloramphenicol to give the fungi the upper hand.
     

    Attached Files:

  19. n0j0y

    n0j0y Well-Known Member

    Joined:
    Nov 19, 2017
    Messages:
    545
    Wasn't sure if some of my old stuff was still living in syringes so just drop a bit on come agar and poof MVIMG_20190310_170741.jpg
     
    MycoFreaK likes this.
  20. Mainframe

    Mainframe Member

    Joined:
    Jan 14, 2019
    Messages:
    86
    Some more cakes are finally pinning. I guess I just didn't expect them to take this long. But anywaysI tried making another small batch of agar and it came out super dark with tons of like shit floating around in it. Maybe it didn't dissolve all the way when mixing? My LC comes out great every time? I'll keep in practicing.