Is this contamination or bruising

Discussion in 'CONTAMINATION' started by Johnny Flash, Sep 27, 2019.

  1. Johnny Flash

    Johnny Flash Active Member

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    Prepared some agar plates growing some Pan Cyan and Golden Teacher spores from shrooms I grew years ago. Do you think the blueing is contam or bruising? See pics.

    Also pic of the Golden Teacher spore agar transfer, onto a fresh agar plate also seeing bluing form as it matures in large rings around the added agar wedge.
     
  2. redcat

    redcat The Myco Machine Moderator Mushroom Doctor

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    Number 1 and 2 look like bluing. Number 3 contam.
     
  3. Johnny Flash

    Johnny Flash Active Member

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    Thanks for your comments, yes I am thinking that you may be correct. As an experiment I have put a few agar slices of number 3 into some sterilized rye grain to see what develops. I know that Pan Cyans especially the ones from Australia blue very easily when handled. I’ll keep you all posted with results.
     
    Last edited: Oct 1, 2019
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  4. LuckOfTheFryish

    LuckOfTheFryish Moderator Moderator Expert Identifier

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    Hard to tell from the pic but it looks to me like #2 could be contam as well
     
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  5. Johnny Flash

    Johnny Flash Active Member

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    Yes trying to work this stuff out can be tricky. The agar is a little dry in my opinion and it could be stress and in number 2 it looks like it is part of the mycelial mass and not a separate entity. Look I’ve put it all into sterilized rye grain and will see what happens. Will post some photos as the grow progresses to help educate the community on things they may encounter. Plate number 3 does look very suspect but also put onto some rye to see what happens.

    The top plate is the result from a wedge taken from the number 2 culture.
    Thanks for the responses.
     
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  6. Johnny Flash

    Johnny Flash Active Member

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    B6AC6F8E-FD7B-4CA5-835B-8588ACC5AEB3.jpeg F36512CE-E82E-4093-B8FA-2687E580A162.jpeg Here are my recently inoculated rye berry jars. The large visible wedge (jar 2) is the Golden Teacher inoculated using the top agar plate no1 in this thread. The top jar is inoculated using the suspect Pan Cyan plate no3.
    I’ll keep updated photos showing growth progress and any contamination. Hopefully there will be no contamination and will get to fruit these projects.
    Cheers Johnny F
     
    Last edited: Oct 1, 2019
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  7. Johnny Flash

    Johnny Flash Active Member

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    Well the mycelium is starting to move and grow in both projects, Top one Pan Cyan and the bottom one Golden Teacher. Will update in a week or so when we have much more growth to see if things remain clean. Cheers Johnny F
     
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  8. Johnny Flash

    Johnny Flash Active Member

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    Well some, not so good news, my two rye berry jars are showing wet spot Contamination. The mycelium is still growing well despite this intruder. No signs of bluing as was evident on my agar plates at start of this thread which tells me that it was simply stress bluing and not a mold contaminant. Will need to do up another couple of rye grain jars to redo the Golden Teacher and Pan Cyan projects. Will still leave these two wet spotted projects going to see if the myc will survive and if I can still get some fruiting done just as a learning segment for our tek community here :)
    Anyway see the latest jar pics to see progress and the wet spot areas.
     
  9. Johnny Flash

    Johnny Flash Active Member

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    Well the Pan Cyan from Australia is very aggressive and the mycelium is overtaking the wet spot contamination. So might still be able to use the grain to inoculate some substrate for fruiting. See updated photo below:
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    The Golden Teacher is having more trouble colonizing the wet spot contam area. But the top half of the jar seems fine. Might still be able to do some fruiting. I will redo these two projects with some fresh grain as I hate contaminated produce. Anyway GT jar is below for your viewing interest.
    0330DF92-F688-4819-ABE4-76EDCF86F74D.jpeg

    Cheers to all, Johnny F
     
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