Liquid Culture

Discussion in 'QUESTIONS & FEEDBACK' started by ElJefeLoco, Apr 11, 2020.

  1. ElJefeLoco

    ElJefeLoco New Member

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    Started a couple last week on the basis of something I read in a book. Now here we are a week later and to be honest, I can't really tell if it's going well or not. That book had only superficial info. Now in order to make sense of what I'm seeing I need something more in depth and stupid-proof. And with lots of pictures :)

    Any ideas?
     
  2. jfost405

    jfost405 Member

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    My liquid culture I use is 5 grams of light corn syrup to 100ml of water. Pc it for 45 min. Cooled and then put a mycelium covered grain in and about week and a bit I had some nice live culture. I found worked better was after I did a grain to grain transfer I kept some colonized grain in the jar. Sterilized some distilled water in syringe in my pc. Injected it in jar once cool. Shake the hell out of it suck it up with the syringe and inoculate your lc mix. But mine took a week and a bit to colonize. I’ll try and snap a pic of what it looks like and hopefully yours will turn out the same
     
  3. jfost405

    jfost405 Member

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    Oh forgot make sure your light corn syrup is mixed well with the water before you pc. Also throw few chunks of glass or a marble in there to break up your LC when you need it. swirl it daily. I do mine 2 times a day. Nothing crazy just enough to get some air bubbles in water but not hard enough for the lc water to touch the lid. upload_2020-4-11_16-57-50.jpeg
     
  4. jfost405

    jfost405 Member

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    upload_2020-4-11_16-58-59.jpeg
     

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  5. jfost405

    jfost405 Member

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  6. jfost405

    jfost405 Member

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    another thing I like is self healing lids on the jars. If you use red rtv silicon (high heat). Drill your hole put the silicon on both sides of the hole and hit wider then it. Then another hole for your vent. I just put micropore tape 2 pieces thick over the vent hole and seems to be good.
     
  7. SleepDeprivedad

    SleepDeprivedad Active Member

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    What size jars you guys use for lc? Only looking for a little 100ml lc but should the jar be a bit larger for shaking and what not... I got I think 8oz or 12oz jars and want to try the rtv but how long do metal lids last for without rusting? Found a bit of rust on a contaminated jar of mine and think it may have been the culprit...
     
  8. jfost405

    jfost405 Member

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    I’m using 16oz jars for 150ml of distilled water but also have 32oz jar made with think was 200 or 250ml in it. The lids last on mine ain’t rusted yet. Guess I should of said spin it to create a vortex effect in it to give air but also try to avoid touching the lid so your air hole that’s filtered won’t get wet. You can get lids pre made on shroom supply website. I haven’t due to on short budget right now.
     
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  9. SleepDeprivedad

    SleepDeprivedad Active Member

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    Thanks duder yea I prolly just shook mine like a crazy person!
     
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  10. jfost405

    jfost405 Member

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    Not a problem I was told that if it touches your filter it’s a good chance it gets contam that way so I avoid it with all costs lol
     
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  11. jfost405

    jfost405 Member

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    That’s my self healing lid the holes same size as the vent one and I just put 2 layers micropore tape on the outside
     
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  12. jfost405

    jfost405 Member

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  13. SleepDeprivedad

    SleepDeprivedad Active Member

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    Easy peasy! My redneck ass has to have rtv somewhere!
     
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  14. MikeLitt

    MikeLitt New Member

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    If you ever have to go to a place to get blood work done. Be very nice, ask the employee for a dozen lavender top tubes for a project with your kid.. L abcorp, Q uest. Are 2 great places. I left a space in the names bc I don't want google showing this to someone. Anyway 3/8 drill bit.. a lid and now you have a proper self healing port. Just did this about 10 minutes ago. 20200430_163955.jpg
     
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  15. jfost405

    jfost405 Member

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    The rtv works great too and is just as good as them ports. Sometimes have To glue them ones in cause they pull out easy. In time no mater what you use a heavy gauge syringe will eat the rubber up and have to replace them rtv just cheap quick route cause can make many jar lids out of one tube of it.
     
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  16. MikeLitt

    MikeLitt New Member

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    I have an unlimited supply, all I had to do was bring the wife lunch. use the employee bathroom and grab a handful of tubes out of the supply closet. my next move is to try to source filter material from same location. :) I am considering grabbing another type tube so I can color reference what that jar is intended for.
     
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  17. JaysayBaylay

    JaysayBaylay New Member

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    Wally world has afew awesome filter materials in the fabric section of Arts and crafts.. Find the batting material(the stuff that fills your quilts and blankets) and they have afew "super thick"-almost exactly like a synthetic filter disc though.. its tough as hell.., it's a white fabric thats $2.68 or 2.78 a yard.. They have like 20 types that could fit that description.. Ill try and see if I have the reciept and item#,.. So basically almost 100 filters or so( if you do the whole top of jar, and there not the wide mouths I suppose too..haha) prob 75-80 of them.. Its legit.. I tried ironing multjple layers of polyfill batting, and was actually relegating myself to that nonsense again, and they didnt have any. So I searched around what they did have, which was plently of batting, and stumbled upon what I feel is a super solid find..
     
  18. JaysayBaylay

    JaysayBaylay New Member

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    I use silicone and a piece of plastic tubing to help form the port, and then cut a piece of the filter stuff about 1/2" bigger than my hole, and seal it on with silicone.. Has stood up to over 10 pc cycles so far.. Tried like 5 diff types of adhesive and was only one that works for multiple runs.. My biggest blunder was using thinking high-heat hot glue would stand up to a 2 hr pc cycle... Hahaha. Was pretty pissed after that pc, when i got all ready to inoculate em the next day and opened it up in my "lab" and realized I had just I fouled up 6 1/2 gallon jars of rye and popcorn like a total tard.. And now wasnt going to be innoculating shit today...haha. Eh ya Live ya learn tho.. Willing to bet I'll prob never brain fart like that again tho.. At least in the Super-near future...hahaha
     
  19. Rockstarr

    Rockstarr New Member

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    Hey, that fogged up when it was growing, right? Then it went clear again?