Liquid culture and hello.

Discussion in 'QUESTIONS & FEEDBACK' started by Binspolicy, Apr 19, 2020.

  1. Binspolicy

    Binspolicy New Member

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    I’m really new to this hobby. And have been researching for the past two weeks. I got hold of some spores (10cc) As all the shops are closed I decided to buy all the parts/bits needed for BRF tek from the net. I got bored of waiting for them all to arrive (just the verm now) and decided to inject some into a liquid culture of Karo and water. As it’s my first ever LC I want some input please. After two days this happened. Small snowflakes.
    how long does liquid culture take to fully form.
    Ps I did sterilise as required.
    Thanks
     

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    Last edited: Apr 20, 2020
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  2. SleepDeprivedad

    SleepDeprivedad Active Member

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    Looks like mycelium... also dont forget to swirl it around to mix up the mycelium. Wait for a nice glob to form then you can start inoculating with it. Waiting is the hardest part of this hobby lol I suck at patience!
     
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  3. Binspolicy

    Binspolicy New Member

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    Thanks for the input man. Feels good to bring something to life from scratch. Already used this to make another jar of LC.
     
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  4. SleepDeprivedad

    SleepDeprivedad Active Member

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    Yea I'm addicted to this hobby now and have soo many ideas of things I want to do lol. Do you have a SAB? And you said you made another LC... from that jar or syringe? That jar wont be ready for a few more days I think but I am very new to all this... keep us posted and check out the needles too short forum I made people have some good things to say.
     
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  5. Binspolicy

    Binspolicy New Member

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    Hi, I don’t have a SAB as of yet. This is all brand new to me and I’m just finding my feet, I will see how things go and possibly make one from a 60 litre tub. Right now I’m practicing as sterile as possible within my means. the new LC was from the “master”? Jar of LC that I had made. I made sure to target one of the snowflakes in the master jar and sucked it into the sterile syringe then injected it into the new jar of 4% Karo. I also made some agar for. Agar and Karo and manages to get a tiny snowflake from the master jar into the dish. Will keep you posted on that too.
    this is really so much fun.
     
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  6. SleepDeprivedad

    SleepDeprivedad Active Member

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    Nice I was hoping you got some mycelium in your syringe! Yea I went without a SAB but failed all 3 of my tubs I did without one so I made one! Use a dremel if you got one to make holes those things like to crack... and I still cracked mine lol! As long as your clean you should be fine!
     
  7. SleepDeprivedad

    SleepDeprivedad Active Member

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    O also a wise man once told me to throw in chunks of broken glass so when you swirl it around the glass with break it apart alot easier and it will colonize alot faster! I also have to not forget to do this lol
     
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  8. jfost405

    jfost405 Member

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    Skebeau I like that last comment haha. But yes looks like good growth. If you don’t have a sab there is another way can do stuff but involves your oven. You heat it up to 200 put a grate like thing you put to ur cookies on when they’re done. Over hang it over the oven opening. Make sure you put something heavy enough to hold it with stuff your working on. Then you put your jars or Petri dishes on it just to say when you open the oven the heat will rise and push dust and whatnot up. Then do your work. Close the oven repeat. I’ve never tried it but seen a guy use that method a few times on YouTube for his agar pours and stuff.
     
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  9. n0j0y

    n0j0y Well-Known Member

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    Can't tell what it is but I'd have concern of anything that formed in 2 days. Generally takes a week for spores to germinate. Possibly sugar crystal from the corn syrup?
     
  10. JaysayBaylay

    JaysayBaylay New Member

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    Could just be fresh spores, good genetics.. Optimal conditions.. Just keep an eye on it.. Just cut the corner of a box and use cyrane(so?) Wrap to cover it.. And cut arm holes in either side.. Total cost of like free if properly sourced.. But def the way to go instead of waiting.. Will usually light up cakes or tear through grains... And then ya cut like a week or so off your estimated time till mushin..or munchin on mush.. Haha. Be careful tho, test it first.. As it sucks huge amounts when ya think its all straight so ya use it, only to just pass that contam into all your hard of spawn... Heart breaking really... Almost wish I took a picture of all those sad empty jars and the garbage bags full of contam grain... Wouldnt wish it on anyone..haha. So since ya have agar, test her there, or use alittle 1/2 pint jar of grain first... Cause LCs are known to hide hidden evil contams, or they jump in along the process... Alls it takes is using an improperly/ not fully sterilized pipette, or. Some such seemingly nonsense and your cookies are burnt... You'll think its all coming along, and then..hmm whats that?!... And if ya dont know, toss it and start over immediately, dont waste time thinking it'll kill it or it'll go away... I mean the first few stages are denial.. And then hysteria... So skip it all and toss it so ya dont waste anymore time, and bridge the gaps in your sterile technique...
     
  11. Nihidao

    Nihidao Active Member

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    Ya def test run I grew out 8 jars of lipstick mold from a swap print .lol .but lc rocks when u get it right. Nojoys right tho that’s fast quit often it can be tamed.but I have heard of spores growing in water if not distilled. Will keep my fingers crossed for ya.
     
  12. Rockstarr

    Rockstarr New Member

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    Got any LC secrets, it does cloud up when its growing, right then it goes clear again like it was when i started?
     
  13. SleepDeprivedad

    SleepDeprivedad Active Member

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    So I think ingot lucky cause I did 3 jars (using shitty baby food jars and the lids seal melted off during sterilization but I just taped it on and it's still going strong)... 2 of the jars did not turn out but I used a clone tissue in very unsterilized environment... I had one jar take off like the happiest mycelium ever! People recommend to use clear kyro or any clear ingredients but I did red potato water with the lighter tan kyro and seems to be fine. After making let sit for 5 to 10 days for a nice glob (people say it looks like jizz and it really does lol). Also if you have a marble or some shards of glass sterilize and put in so after they glob starts you can swirl around without opening it just agitate it and the marble or glass will help break mycelium.... I didnt dont but it's still breaking up nicely. Also swirl nicely before trying to pull from it. If i missed something let me know!
     
  14. Binspolicy

    Binspolicy New Member

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    I read on Shroomery you can use sealed lids as LC dont need much air. I can confirm it works. Then you can just violently shake the jar. No need for marble etc.
     
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  15. SleepDeprivedad

    SleepDeprivedad Active Member

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    Where do you do your sterilized draw from? Do you just remove lid? I dont think I like that lol too many cats! I am gonna try the rtv sealed lid from the tek on here then you don't have to hassle with tape!
     
  16. Binspolicy

    Binspolicy New Member

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    Yup just tilt the jar slightly remove lid. Draw up 20cc and bam. Easy really
     
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  17. SleepDeprivedad

    SleepDeprivedad Active Member

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    Right I hear alot of hate about LC I understand you cannot tell if there is contam present but they shit just grows and grows lol how can you not love it? It saved me when I thought all was lost due to my last tub i was gonna print from got consumed by Trichoderma...looked in closet and there was my glob of lc waiting to be colonized onto something!
     
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  18. GGTBod

    GGTBod Chronicles of Bodley Administrator Cannabis Doctor Cacti Doctor

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    I kinda feel LC is a waste of time, the idea of the LC being is it's a bit of a jump start from a spore print or culture on agar, but, the very first thing i gotta do if i buy an LC is running it on agar for a couple of weeks to make sure it is clean, it's no good knocking up 100 jars of grain and finding out 3 weeks later they all contam cos your LC was dirty, so as soon as i get an LC it's a couiple of drips from each vial or syringe onto agar plates and then waiting a couple of weeks to check no contams grow, then if clean it goes onto grain jars, so the illusion of any time saved is just that, an illusion.

    If you making them yourself for yourself in front of a flow hood, different matter, as you can verify to yourself they are clean.

    :hatsoff2:
     
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  19. SleepDeprivedad

    SleepDeprivedad Active Member

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    I need to dial in my agar game for sure plus I heard you can use slants for long term storage which isnt it similar ingredients as agar? How long does lc take to germinate agar... mine arent working but I am going to try the no pour tek next time... do you mark where you drip lc on agar or just wait and watch? Have read about the spore loop but I was wondering about just dripping so thanks bod that helps... yes I use lc for my own ass saving lol and I wish I could do up 100 jars I get lucky to do 6.... someday I will be a god like bod!

    Also stupid question but if you get one drop on agar from lc that's clean does that mean the whole jars is clean or did you just get lucky and baddies may still be lurking?
     
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  20. GGTBod

    GGTBod Chronicles of Bodley Administrator Cannabis Doctor Cacti Doctor

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    No gods here mate, just creatures of the mycelium :D cheers though hahaha i'm just farther down the same road that you are on yourself.

    For the drops on agar i just wait for action which you should see in a few days, if the lc grows clean mycelim on a again plate, then we will often do a transfer of the most ropey looking parts onto new agar dishes/no pour jars to expand it, if clean once in the expanded plates we then just innoculate grain jars from the agar plate as we know it's clean, the LC is kept and labled as clean?

    In the start of this vid we are doing just that, from petri plates to no pour jars without a flow hood



    For slants it's just agar with additives usually, like a popsicle stick for the woodies
     
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